Department of Physics, University of Messina, 98165 Messina, Italy.
Oxid Med Cell Longev. 2013;2013:414393. doi: 10.1155/2013/414393. Epub 2013 Jul 16.
SH-SY5Y neuroblastoma cells were used as an experimental model to study the effects of 50 Hz electromagnetic field, in the range from 50 µ T to 1.4 mT. Fourier transform infrared spectroscopy analysis evidenced a reduction in intensity of the amide A band and a slight increase of vibration bands at 2921 cm(-1) and 2853 cm(-1) corresponding to methylene groups. A further increase of the magnetic field intensity of exposure up to 0.8 mT and 1.4 mT produced a clear increase in intensity of CH2 vibration bands. Moreover, it has been observed some alterations in the amide I region, such as a shifted peak of the amide I band to a smaller wavenumber, probably due to protein conformational changes. These results suggested that exposure to extremely low electromagnetic fields influenced lipid components of cellular membrane and the N-H in-plane bending and C-N stretching vibrations of peptide linkages, modifying the secondary structures of α -helix and β -sheet contents and producing unfolding process in cell membrane proteins. The observed changes after exposure to 50 Hz electromagnetic field higher than 0.8 mT were associated with a significant reduction of cell viability and reduced mitochondrial transmembrane potential.
SH-SY5Y 神经母细胞瘤细胞被用作实验模型，以研究 50 Hz 电磁场的影响，范围为 50 µT 至 1.4 mT。傅里叶变换红外光谱分析表明酰胺 A 带的强度降低，2921 cm(-1)和 2853 cm(-1)处对应于亚甲基基团的振动带略有增加。进一步增加暴露于磁场的强度至 0.8 mT 和 1.4 mT，导致 CH2 振动带的强度明显增加。此外，还观察到酰胺 I 区域的一些变化，例如酰胺 I 带的峰向较小的波数移动，这可能是由于蛋白质构象发生变化。这些结果表明，暴露于极低频电磁场会影响细胞膜的脂质成分以及肽键中的 N-H 面内弯曲和 C-N 伸缩振动，从而改变α-螺旋和β-折叠含量的二级结构，并导致细胞膜蛋白的展开过程。暴露于高于 0.8 mT 的 50 Hz 电磁场后观察到的变化与细胞活力的显著降低和线粒体跨膜电位的降低有关。
