Zduńczyk Z, Drazbo A, Jankowski J, Juśkiewicz J, Czech A, Antoszkiewicz Z
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
Pol J Vet Sci. 2013;16(2):333-9. doi: 10.2478/pjvs-2013-0045.
The effect of different dietary levels of selenium (Se) and vitamin E on egg production, the antioxidant status and the immune system response of hens was investigated in the current study. A total of 32 Lohman Brown hens were divided into four groups and were fed diets with 5% of soybean oil and two levels of Se (0.15 and 0.30 mg/kg) and vitamin E (30 and 60 mg/kg). During 10 weeks of experimental feeding, the body weights of hens and egg production were similar in all dietary treatments, but a higher Se content of diets contributed to a significant increase in egg weight. A higher vitamin E level significantly increased alpha-tocopherol concentrations (2.71 vs. 2.05 microg/mL, p = 0.001), superoxide dismutase (SOD) activity (43.3 vs. 39.9 U/mL, p = 0.049) and the ferric reducing ability of serum (FRAP) (123.0 vs. 105.7 micromol/L, p = 0.029). A higher Se content increased the concentrations of ascorbic acid (0.309 vs. 0.073 microg/L, p = 0.001), retinol (1.48 vs. 1.15 microg/mL, p = 0.001) and alpha-tocopherol (2.86 vs. 1.90 microg/mL, p = 0.001), the activity levels of catalase (3.40 vs. 2.98 U/L,p = 0.010) and SOD (43.4 vs. 30.8 U/mL, p = 0.040) as well as the total antioxidant status (TAS) of serum (0.38 vs. 0.28 mmol/L, p = 0.026). There were no significant differences in the concentrations of tumor necrosis factor-alpha (TNF-a) and interleukin-6 (IL-6) between treatments, while a higher vitamin E content of diets increased serum immunoglobulin A (IgA) concentrations (370.6 vs. 321.3 microg/mL, p = 0.026). Higher dietary levels of vitamin E and Se increased the serum concentrations of retinol (1.66 vs. 2.20 microg/mL, p = 0.013) and a-tocopherol (3.33 vs. 1.71, p = 0.014), but they had no effect on the other physiological parameters. It is recommended that the levels of both supplements, Se and vitamin E, be increased in laying hen diets as they have a beneficial effect on the serum concentrations of retinol and alpha-tocopherol.
本研究调查了不同日粮水平的硒（Se）和维生素E对母鸡产蛋性能、抗氧化状态及免疫系统反应的影响。将32只罗曼褐母鸡分为四组，饲喂含5%大豆油以及两种硒水平（0.15和0.30毫克/千克）和维生素E水平（30和60毫克/千克）的日粮。在10周的试验饲养期内，所有日粮处理组母鸡的体重和产蛋量相似，但日粮中较高的硒含量使蛋重显著增加。较高的维生素E水平显著提高了α-生育酚浓度（2.71对2.05微克/毫升，p = 0.001）、超氧化物歧化酶（SOD）活性（43.3对39.9单位/毫升，p = 0.049）以及血清铁还原能力（FRAP）（123.0对105.7微摩尔/升，p = 0.029）。较高的硒含量增加了抗坏血酸浓度（0.309对0.073微克/升，p = 0.001）、视黄醇浓度（1.48对1.15微克/毫升，p = 0.001）和α-生育酚浓度（2.86对1.90微克/毫升，p = 0.001），过氧化氢酶（3.40对2.98单位/升，p = 0.010）和SOD的活性水平（43.4对30.8单位/毫升，p = 0.040）以及血清总抗氧化状态（TAS）（0.38对0.28毫摩尔/升，p = 0.026）。各处理组之间肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的浓度无显著差异，而日粮中较高的维生素E含量增加了血清免疫球蛋白A（IgA）浓度（370.6对321.3微克/毫升，p = 0.026）。日粮中较高水平的维生素E和硒增加了血清视黄醇浓度（1.66对2.20微克/毫升，p = 0.013）和α-生育酚浓度（3.33对1.71，p = 0.014），但对其他生理参数无影响。建议在蛋鸡日粮中同时提高硒和维生素E这两种添加剂的水平，因为它们对血清视黄醇和α-生育酚浓度有有益影响。
