Institute for Stem Cell Biology and Regenerative Medicine, NCBS-TIFR , Bangalore, 560065 India.
ACS Chem Biol. 2014 Jan 17;9(1):147-55. doi: 10.1021/cb400486t. Epub 2013 Sep 11.
Cell-cell communications, cell-matrix interactions, and cell migrations play a major role in regeneration. However, little is known about the molecular players involved in these critical events, especially cell surface molecules. Here, we demonstrate the role of specific glycan-receptor interactions in the regenerative process using Hydra magnipapillata as a model system. Global characterization of the N- and O-glycans expressed by H. magnipapillata using ultrasensitive mass spectrometry revealed mainly polyfucosylated LacdiNAc antennary structures. Affinity purification showed that a putative C-type lectin (accession number Q6SIX6) is a likely endogenous receptor for the novel polyfucosylated glycans. Disruption of glycan-receptor interactions led to complete shutdown of the regeneration machinery in live Hydra. A time-dependent, lack-of-regeneration phenotype observed upon incubation with exogenous fuco-lectins suggests the involvement of a polyfucose receptor-mediated signaling mechanism during regeneration. Thus, for the first time, the results presented here provide direct evidence for the role of polyfucosylated glycan-receptor interactions in the regeneration of H. magnipapillata.
细胞间通讯、细胞基质相互作用和细胞迁移在再生中起着重要作用。然而，对于参与这些关键事件的分子参与者，特别是细胞表面分子，我们知之甚少。在这里，我们使用 Hydra magnipapillata 作为模型系统，证明了特定糖受体相互作用在再生过程中的作用。使用超灵敏质谱法对 H. magnipapillata 表达的 N-和 O-聚糖进行全面表征，结果主要显示多岩藻糖基化 LacdiNAc 天线结构。亲和纯化表明，一种假定的 C 型凝集素（登录号 Q6SIX6）可能是新型多岩藻糖基化聚糖的内源性受体。糖受体相互作用的破坏导致活体 Hydra 再生机制完全关闭。在用外源岩藻糖凝集素孵育时观察到的时间依赖性无再生表型表明，在再生过程中涉及多岩藻糖受体介导的信号机制。因此，本文首次提供了直接证据，证明多岩藻糖基化糖受体相互作用在 Hydra magnipapillata 的再生中的作用。
