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桃胶衍生低聚糖的抗氧化活性和抗菌活性

Antioxidant activity and antibacterial activity of peach gum derived oligosaccharides.

作者信息

Yao Xing-Cun, Cao Yan, Wu Sheng-Jun

机构信息

School of Food Engineering, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China.

出版信息

Int J Biol Macromol. 2013 Nov;62:1-3. doi: 10.1016/j.ijbiomac.2013.08.022. Epub 2013 Aug 22.

Abstract

In this study, peach gum derived oligosaccharides (PGDO) were prepared from peach gum polysaccharides by hydrolysis using hydrogen peroxide (H2O2) under following conditions: time, 8h; temperature, 55 °C; H2O2 concentration, 4% (v/v); and NaOH concentration, 2.0 M. The antioxidant activity and antibacterial activity of PGDO were estimated. There were no significant chemical changes in the backbones of the peach gum polysaccharides treated with H2O2. The PGDO showed high hydroxyl radical scavenging activity (86.12%) and 2, 2-diphenyl-β-picrylhydrazyl radical scavenging activity (91.70%) at the concentration of 100 μg/mL as well as high reducing capacity at the concentration of 50 μg/mL. In addition the PGDO had high antibacterial activity against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli at the concentration of 100 μg/mL.

摘要

在本研究中,桃胶衍生的低聚糖(PGDO)是通过在以下条件下用过氧化氢(H₂O₂)水解桃胶多糖制备的:时间为8小时;温度为55℃;H₂O₂浓度为4%(v/v);NaOH浓度为2.0 M。对PGDO的抗氧化活性和抗菌活性进行了评估。用H₂O₂处理的桃胶多糖主链没有显著的化学变化。PGDO在浓度为100μg/mL时表现出较高的羟基自由基清除活性(86.12%)和2,2-二苯基-β-苦基肼自由基清除活性(91.70%),在浓度为50μg/mL时具有较高的还原能力。此外,PGDO在浓度为100μg/mL时对枯草芽孢杆菌、金黄色葡萄球菌和大肠杆菌具有较高的抗菌活性。

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