Laboratoire SONAS, Université d'Angers, SFR QUASAV, Angers 49045, France.
Molecules. 2013 Aug 22;18(9):10213-27. doi: 10.3390/molecules180910213.
The aim of this study was to develop faster and more efficient phenotyping methods for in-depth genetic studies on cider apple progeny. The UHPLC chromatographic system was chosen to separate polyphenolic compounds, and quantifications were then simultaneously performed with a UV-PDA detector and an ESI-triple quadrupole mass analyzer (SRM mode). Both quantification methods were validated for 15 major compounds using two apple juice samples, on the basis of linearity, limits of detection and quantification, recovery and precision tests. The comparison between UV and SRM quantifications in 120 different samples of a cider apple progeny showed an excellent correlation for major compounds quantified with both methods. However, an overestimation was revealed for five compounds with the UV detector and the mass analyzer. Co-elution and matrix effects are discussed to explain this phenomenon. SRM methods should therefore be considered with restrictions in some cases for quantification measurements when several phenolic compounds are simultaneously quantified in complex matrices such as apple juices. For both methods, analyses were carried out over short periods of time while maintaining a high quality for the simultaneous quantification of phenolic compounds in apple juice. Each method is relevant for more in-depth genetic studies of the polyphenol content of apple juice.
本研究旨在开发更快、更有效的 cider apple 后代深入遗传研究的表型分析方法。选择 UHPLC 色谱系统分离多酚化合物，然后用 UV-PDA 检测器和 ESI-三重四极杆质谱仪（SRM 模式）同时进行定量分析。两种定量方法均基于苹果汁样品的线性、检测限和定量限、回收率和精密度试验对 15 种主要化合物进行了验证。在 cider apple 后代的 120 个不同样本中，对两种方法定量的主要化合物进行比较，结果表明，两种方法的相关性极好。然而，用紫外检测器和质谱仪对五种化合物进行定量时，发现存在高估现象。本文讨论了共洗脱和基质效应来解释这一现象。因此，在某些情况下，当同时对复杂基质（如苹果汁）中的多种酚类化合物进行定量测量时，应考虑使用 SRM 方法。对于这两种方法，在短时间内进行分析，同时保持对苹果汁中酚类化合物的定量分析的高质量。每种方法都适用于更深入的苹果汁多酚含量的遗传研究。
