Jegou B, Dacheux J L, Terqui M, Garnier D H, Courot M
Mol Cell Endocrinol. 1978 Jan;9(3):335-46. doi: 10.1016/0303-7207(78)90075-8.
An androgen binding protein (ABP) with an electrophoretic mobility (Rf) of 0.56 is present in the rete testis fluid of adult rams. Its steroid specificity was found to be in the following order: 5alpha-DHT, testosterone, oestradiol-17 beta, dehydroepiandrosterone 5beta-DHT, androstenedione, cyproterone, cyproterone acetate, cortisol and progesterone. The characteristics of the ABP are similar to those found for the ABP of the testis and the epididymis of the rat and the rabbit. The concentration of ABP, determined by the dextran-coated charcoal method and sometimes confirmed by the steady-state polyacrylamide gel electrophoresis method, was significantly higher in the breeding season than in the non-breeding season (4.40 +/- 0.98 X 10(-9) M vs. 2.60 +/- 0.62 X 10(-9) M; P less than 0.037). The affinity constant of the ABP was independent of the season (2.45 +/- 0.21 X 10(9) M-1 vs. 2.66 +/- 0.1 X 10(9) M-1; NS). In addition, ABP was positively correlated with 5alpha-DHT (r = 0.506; P less than 0.0009), testosterone (r = 0.445; P less than 0.0003), total protein (r = 0.329; P less than 0.02) and spermatozoa (r = 0.406; P less than 0.006) in the RTF and with blood plasma testosterone (r = 0.584; P less than 0.0001). Furthermore, testosterone and 5alpha-DHT in RTF were positively correlated (r = 0.582; P less than 0.0001). These androgens were also correlated with plasma testosterone (r = 0.262, P less than 0.052 for testosterone in RTF; r = 0.341, P less than 0.018 for 5 alpha-DHT). Total proteins and spermatozoa were found to be positively correlated in the RTF (r = 0.789; P less than 0.0001).
成年公羊的睾丸网液中存在一种电泳迁移率（Rf）为0.56的雄激素结合蛋白（ABP）。发现其类固醇特异性顺序如下：5α-双氢睾酮、睾酮、雌二醇-17β、脱氢表雄酮、5β-双氢睾酮、雄烯二酮、环丙孕酮、醋酸环丙孕酮、皮质醇和孕酮。该ABP的特性与在大鼠和兔子的睾丸及附睾中发现的ABP相似。通过葡聚糖包被活性炭法测定且有时通过稳态聚丙烯酰胺凝胶电泳法确认的ABP浓度，在繁殖季节显著高于非繁殖季节（4.40±0.98×10⁻⁹ M对2.60±0.62×10⁻⁹ M；P＜0.037）。ABP的亲和常数与季节无关（2.45±0.21×10⁹ M⁻¹对2.66±0.1×10⁹ M⁻¹；无显著性差异）。此外，睾丸网液中的ABP与5α-双氢睾酮（r = 0.506；P＜0.0009）、睾酮（r = 0.445；P＜0.0003）、总蛋白（r = 0.329；P＜0.02）和精子（r = 0.406；P＜0.006）呈正相关，且与血浆睾酮呈正相关（r = 0.584；P＜0.0001）。此外，睾丸网液中的睾酮和5α-双氢睾酮呈正相关（r = 0.582；P＜0.0001）。这些雄激素也与血浆睾酮相关（睾丸网液中的睾酮r = 0.262，P＜0.052；5α-双氢睾酮r = 0.341，P＜0.018）。发现睾丸网液中的总蛋白和精子呈正相关（r = 0.789；P＜0.0001）。
