Davis F C, Boada R, LeDeaux J
Department of Biology, Northeastern University, Boston, MA 02115.
Brain Res. 1990 Jun 11;519(1-2):192-9. doi: 10.1016/0006-8993(90)90077-o.
Neurogenesis of the hypothalamic suprachiasmatic nucleus (SCN) was described in the Syrian hamster (Mesocricetus auratus) using tritiated [3H]thymidine autoradiography. Pregnant hamsters were given single intraperitoneal injections of [3H]thymidine at different times during prenatal development, and labeled cells were analyzed in the offspring of 4-5 weeks of age. Cells of the hamster SCN became postmitotic (were 'born') over two and a half days from 10.5 to 13.0 days postfertilization (dpf) with a peak around 11.5 dpf, 4 days before birth. Two gradients in SCN neurogenesis were observed. Posterior cells were produced somewhat earlier than anterior cells and ventrolateral cells were produced before dorsomedial cells. An exception to the second gradient was a small population of ventrolateral cells produced near the end of SCN neurogenesis. The pattern of SCN neurogenesis in the hamster was similar to that described in the rat, including a predominant ventrolateral to dorsomedial gradient and the presence of ventral or ventrolateral cells produced relatively late, contrary to the predominant gradient.
利用氚化[³H]胸腺嘧啶核苷放射自显影技术，在叙利亚仓鼠（金仓鼠）中描述了下丘脑视交叉上核（SCN）的神经发生。在产前发育的不同时间，给怀孕的仓鼠腹腔内单次注射[³H]胸腺嘧啶核苷，并对4 - 5周龄后代中的标记细胞进行分析。仓鼠SCN的细胞在受精后10.5至13.0天的两天半时间内进入有丝分裂后期（即“出生”），在出生前4天左右的11.5 dpf达到峰值。观察到SCN神经发生存在两个梯度。后部细胞的产生略早于前部细胞，腹外侧细胞的产生早于背内侧细胞。第二个梯度的一个例外是在SCN神经发生接近尾声时产生的一小群腹外侧细胞。仓鼠SCN神经发生的模式与在大鼠中描述的相似，包括从腹外侧到背内侧的主要梯度以及相对较晚产生的腹侧或腹外侧细胞的存在，这与主要梯度相反。
