Institute of Neuroanatomy; Universitätsmedizin Göttingen; Göttingen, Germany.
Cell Cycle. 2013 Sep 15;12(18):2953-9. doi: 10.4161/cc.25999. Epub 2013 Aug 13.
The multi-subunit chromatin remodeling BAF complex controls different developmental processes. Using cortex-specific conditional knockout and overexpression mouse models, we have recently reported that BAF170, a subunit of the vertebrate BAF chromatin remodeling complex, interacts with transcription factor (TF) Pax6 to control cortical size and volume. The mechanistic basis includes suppression of the expression of Pax6 target genes, which are required for genesis of cortical intermediate progenitors (IPs) and specification of late neuronal subtype identity. In addition, we showed that a dynamic competition between BAF170 and BAF155 subunits within the BAF complex during progression of neurogenesis is a primary event in modulating the size of the mammalian cortex. Here, we present additional insights into the interaction between the BAF complex and TF Pax6 in the genesis of IPs of the developing cortex. Furthermore, we show that such competition between BAF170 and BAF155 is involved as well in the determination of the size of the embryonic body. Our results add new insights into a cell-intrinsic mechanism, mediated by the chromatin remodeling BAF complex that controls vertebrate body shape and size.
多亚基染色质重塑 BAF 复合物控制着不同的发育过程。我们最近使用皮质特异性条件敲除和过表达小鼠模型报告称，脊椎动物 BAF 染色质重塑复合物的一个亚基 BAF170 与转录因子 (TF) Pax6 相互作用，以控制皮质大小和体积。其机制基础包括抑制 Pax6 靶基因的表达，这些基因对于皮质中间祖细胞 (IPs) 的发生和晚期神经元亚型身份的特化是必需的。此外，我们还表明，在神经发生过程中，BAF 复合物内 BAF170 和 BAF155 亚基之间的动态竞争是调节哺乳动物皮质大小的主要事件。在这里，我们提供了 BAF 复合物与 TF Pax6 之间在发育中的皮质 IP 生成中的相互作用的更多见解。此外，我们还表明，BAF170 和 BAF155 之间的这种竞争也参与了胚胎体大小的决定。我们的结果为染色质重塑 BAF 复合物控制脊椎动物体型和大小的细胞内在机制提供了新的见解。
