State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang, 330047, Jiangxi, People's Republic of China,
Biotechnol Lett. 2013 Dec;35(12):2147-53. doi: 10.1007/s10529-013-1319-y. Epub 2013 Aug 24.
Candida albicans is an opportunistic human pathogen whose disinfection is a challenge. ε-Poly-L-lysine (ε-PL), an antagonistic agent, can disrupt cell membranes and inhibit the growth of C. albicans. Genes that were differentially-expressed in response to ε-PL were isolated from C. albicans and identified by suppression subtractive hybridization. Ten subtracted clones, that share >98 % homology with known genes of C. albicans, were isolated. Among these, four genes encoded cell wall-associated proteins. Real-time quantitative PCR and northern blot hybridization suggest that these genes are involved in the response to ε-PL. These findings will help to determine the mechanism of the antimicrobial activity of ε-PL against C. albicans.
白色念珠菌是一种机会性人类病原体，其消毒是一个挑战。ε-聚赖氨酸（ε-PL）是一种拮抗试剂，可破坏细胞膜并抑制白色念珠菌的生长。通过抑制差减杂交，从白色念珠菌中分离出响应ε-PL 表达差异的基因，并进行鉴定。从白色念珠菌中分离出 10 个与已知基因具有>98％同源性的消减克隆。其中，有 4 个基因编码细胞壁相关蛋白。实时定量 PCR 和 northern blot 杂交表明，这些基因参与了对 ε-PL 的反应。这些发现将有助于确定 ε-PL 对白色念珠菌的抗微生物活性的机制。
