Aizawa S, Sado T
Division of Physiology and Pathology, National Institute of Radiological Sciences, Chiba, Japan.
Cell Immunol. 1990 Oct 1;130(1):1-10. doi: 10.1016/0008-8749(90)90156-l.
Previous study has demonstrated that a considerable number of antigen-specific, allo H-2-restricted CTLPs could be recovered from normal lymphocytes. Experiments were conducted to examine whether antigen-specific, self H-2-restricted T cells could also manifest allo H-2-restriction specificity. Non-H-2-specific, self H-2-restricted CTLs were induced in the microcultures with a limited number of responder spleen cells from B10(H-2b) mice that had been primed with C3H.SW(H-2b) spleen cells. Two hundred thirteen non-H-2-specific CTLs generated in limiting dilution culture condition were assayed for cross-reactivity against TNP-modified and unmodified allogeneic targets of 10 different H-2 haplotypes. Of these CTLs, 29 (14%) were cross-reactive to a TNP-modified allogeneic target and seventeen (8%) to an unmodified allogeneic target. The quantitative analysis for cross-reactivity of non-H-2 specific, self H-2-restricted CTLs reveals a significant number of CTLs that were cross-reactive with either alloantigen or antigen (TNP) plus allo H-2. Furthermore, CTL clones with triple specificities including self H-2-restriction specificity, allo-reactivity, and allo H-2 restriction specificity were also found. These results indicate that antigen-specific, allo H-2-restricted T cells are distinct not only from alloreactive T cells but also from antigen-specific, self H-2-restricted T cells. Significance of the manifestation of allo H-2 restriction specificity by self H-2-restricted T cells is discussed in regard to the generation of T cell repertoire.
先前的研究表明，从正常淋巴细胞中可以分离出相当数量的抗原特异性、同种异体H-2限制性CTL前体细胞。进行实验以检查抗原特异性、自身H-2限制性T细胞是否也能表现出同种异体H-2限制性特异性。用有限数量的来自已用C3H.SW（H-2b）脾细胞致敏的B10（H-2b）小鼠的反应性脾细胞在微量培养物中诱导出非H-2特异性、自身H-2限制性CTL。对在有限稀释培养条件下产生的213个非H-2特异性CTL进行检测，以观察它们对10种不同H-2单倍型的TNP修饰和未修饰的同种异体靶标的交叉反应性。在这些CTL中，29个（14%）对TNP修饰的同种异体靶标有交叉反应，17个（8%）对未修饰的同种异体靶标有交叉反应。对非H-2特异性、自身H-2限制性CTL交叉反应性的定量分析显示，有相当数量的CTL与同种异体抗原或抗原（TNP）加同种异体H-2有交叉反应。此外，还发现了具有三重特异性的CTL克隆，包括自身H-2限制性特异性、同种异体反应性和同种异体H-2限制性特异性。这些结果表明，抗原特异性、同种异体H-2限制性T细胞不仅与同种异体反应性T细胞不同，而且与抗原特异性、自身H-2限制性T细胞也不同。关于T细胞库的产生，讨论了自身H-2限制性T细胞表现出同种异体H-2限制性特异性的意义。
