Akbar Mohammed Touaha, Habib Abdul Musaweer, Chowdhury Dil Umme Salma, Bhuiyan Md Iqbal Kaiser, Mostafa Kazi Md Golam, Mondol Sobuj, Mosleh Ivan Mhai
Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong - 4331, Bangladesh.
Bioinformation. 2013 Aug 7;9(14):730-5. doi: 10.6026/97320630009730. eCollection 2013.
Macrophomina phaseolina is one of the deadliest necrotrophic fungal pathogens that infect more than 500 plant species including major food, fiber, and oil crops all throughout the globe. It secretes a cocktail of ligninolytic enzymes along with other hydrolytic enzymes for degrading the woody lignocellulosic plant cell wall and penetrating into the host tissue. Among them, lignin peroxidase has been reported only in Phanerochaete chrysosporium so far. But interestingly, a recent study has revealed a second occurrence of lignin peroxidase in M. phaseolina. However, lignin peroxidases are of much significance biotechnologically because of their potential applications in bio-remedial waste treatment and in catalyzing difficult chemical transformations. Besides, this enzyme also possesses agricultural and environmental importance on account of their role in lignin biodegradation. In the present work, different properties of the lignin peroxidase of M. phaseolina along with predicting the 3-D structure and its active sites were investigated by the use of various computational tools. The data from this study will pave the way for more detailed exploration of this enzyme in wet lab and thereby facilitating the strategies to be designed against such deadly weapons of Macrophomina phaseolina. Furthermore, the insight of such a ligninolytic enzyme will contribute to the assessment of its potentiality as a bioremediation tool.
菜豆壳球孢菌是最致命的坏死营养型真菌病原体之一，可感染全球500多种植物，包括主要的粮食、纤维和油料作物。它会分泌一系列木质素分解酶以及其他水解酶，用于降解木质纤维素植物细胞壁并侵入宿主组织。其中，迄今为止仅在黄孢原毛平革菌中报道过木质素过氧化物酶。但有趣的是，最近一项研究发现菜豆壳球孢菌中也存在木质素过氧化物酶。然而，木质素过氧化物酶在生物技术方面具有重要意义，因为它们在生物修复废物处理和催化困难的化学转化方面具有潜在应用。此外，由于该酶在木质素生物降解中的作用，它在农业和环境方面也具有重要意义。在本研究中，利用各种计算工具研究了菜豆壳球孢菌木质素过氧化物酶的不同性质，并预测了其三维结构及其活性位点。本研究的数据将为在湿实验室中更详细地探索这种酶铺平道路，从而有助于设计针对菜豆壳球孢菌这种致命武器的策略。此外，对这种木质素分解酶的深入了解将有助于评估其作为生物修复工具的潜力。
