University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.
PLoS One. 2013 Aug 15;8(8):e71397. doi: 10.1371/journal.pone.0071397. eCollection 2013.
Recent application of gene expression profiling to the immune system has shown a great potential for characterization of complex regulatory processes. It is becoming increasingly important to characterize functional systems through multigene interactions to provide valuable insights into differences between healthy controls and autoimmune patients. Here we apply an original systematic approach to the analysis of changes in regulatory gene interconnections between in Epstein-Barr virus transformed hyperresponsive B cells from SLE patients and normal control B cells. Both traditional analysis of differential gene expression and analysis of the dynamics of gene expression variations were performed in combination to establish model networks of functional gene expression. This Pathway Dysregulation Analysis identified known transcription factors and transcriptional regulators activated uniquely in stimulated B cells from SLE patients.
最近，基因表达谱在免疫系统中的应用显示出了在描述复杂调控过程方面的巨大潜力。通过多基因相互作用来描述功能系统变得越来越重要，这可以为健康对照和自身免疫患者之间的差异提供有价值的见解。在这里，我们应用一种原始的系统方法来分析来自 SLE 患者的 Epstein-Barr 病毒转化的高反应性 B 细胞与正常对照 B 细胞之间调节基因相互连接的变化。传统的差异基因表达分析和基因表达变化动态分析相结合，建立了功能基因表达的模型网络。这种途径失调分析确定了在 SLE 患者刺激的 B 细胞中独特激活的已知转录因子和转录调节剂。
