Department of Biological & Environmental Engineering, ∥Department of Molecular Biology and Genetics, and ⊥Kavli Institute at Cornell for Nanoscale Science, Cornell University , Ithaca, New York 14853, United States.
J Am Chem Soc. 2013 Sep 25;135(38):14008-11. doi: 10.1021/ja405872g. Epub 2013 Sep 11.
Protein immune detection requires secondary antibodies which must be carefully selected in order to avoid interspecies cross-reactivity, and is therefore restricted by the limited availability of primary/secondary antibody pairs. Here we present a versatile DNA-based protein detection system using a universal adapter to interface between IgG antibodies and DNA-modified reporter molecules. As a demonstration of this capability, we successfully used DNA nano-barcodes, quantum dots, and horseradish peroxidase enzyme to detect multiple proteins using our DNA-based labeling system. Our system not only eliminates secondary antibodies but also serves as a novel method platform for protein detection with modularity, high capacity, and multiplexed capability.
蛋白质免疫检测需要二级抗体，为了避免种间交叉反应，必须仔细选择，因此受到一级/二级抗体对有限可用性的限制。在这里，我们提出了一种基于 DNA 的通用蛋白质检测系统，该系统使用通用接头将 IgG 抗体与 DNA 修饰的报告分子连接起来。作为这种能力的证明，我们成功地使用 DNA 纳米条形码、量子点和辣根过氧化物酶酶使用我们的基于 DNA 的标记系统来检测多种蛋白质。我们的系统不仅消除了二级抗体，而且还作为一种具有模块化、大容量和多重能力的新型蛋白质检测方法平台。
