Souza Rimenez R, França Sanmara L, Bessa Marília M, Takahashi Reinaldo N
Departamento de Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Behav Processes. 2013 Nov;100:67-73. doi: 10.1016/j.beproc.2013.08.008. Epub 2013 Aug 23.
Due to the ability for depleting neuronal storages of monoamines, the reserpine model is a suitable approach for the investigation of the neurobiology of neurodegenerative diseases. However, the behavioral effects of low doses of reserpine are not always detected by classic animal tests of cognition, emotion, and sensory ability. In this study, the effects of reserpine (0.5-1.0mg/kg) were evaluated in olfactory fear conditioning, inhibitory avoidance, open-field, elevated plus-maze, and olfactory discrimination. Possible protective effects were also investigated. We found that single administration of reserpine impaired the acquisition of olfactory fear conditioning (in both doses) as well as olfactory discrimination (in the higher dose), while no effects were seen in all other tests. Additionally, we demonstrated that prior exposure to environmental enrichment prevented effects of reserpine in animals tested in olfactory fear conditioning. Altogether, these findings suggest that a combined cognitive, emotional and sensory-dependent task would be more sensitive to the effects of the reserpine model. In addition, the present data support the environmental enrichment as an useful approach for the study of resilience mechanisms in neurodegenerative processes.
由于利血平具有耗尽神经元单胺储存的能力，利血平模型是研究神经退行性疾病神经生物学的合适方法。然而，低剂量利血平的行为效应并不总是能通过经典的动物认知、情感和感觉能力测试检测到。在本研究中，我们评估了利血平（0.5 - 1.0mg/kg）在嗅觉恐惧条件反射、抑制性回避、旷场、高架十字迷宫和嗅觉辨别测试中的作用。我们还研究了其可能的保护作用。我们发现，单次给予利血平会损害嗅觉恐惧条件反射（两种剂量均有此作用）以及嗅觉辨别（高剂量时），而在所有其他测试中未观察到影响。此外，我们证明，预先暴露于丰富环境可防止利血平对进行嗅觉恐惧条件反射测试的动物产生影响。总之，这些发现表明，一项结合认知、情感和感觉依赖的任务对利血平模型的效应更敏感。此外，目前的数据支持丰富环境作为研究神经退行性过程中恢复力机制的一种有用方法。
