Brandão Luiz Eduardo Mateus, Nôga Diana Aline Morais Ferreira, Dierschnabel Aline Lima, Campêlo Clarissa Loureiro das Chagas, Meurer Ywlliane da Silva Rodrigues, Lima Ramón Hypolito, Engelberth Rovena Clara Galvão Januário, Cavalcante Jeferson Souza, Lima Clésio Andrade, Marchioro Murilo, Estevam Charles Dos Santos, Santos José Ronaldo, Silva Regina Helena, Ribeiro Alessandra Mussi
Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
Universidade Federal de Sergipe, São Cristóvão, SE, Brazil.
Evid Based Complement Alternat Med. 2017;2017:8429290. doi: 10.1155/2017/8429290. Epub 2017 Aug 1.
Masters is a Brazilian native species of passionflower. This genus is known in the American continent folk medicine for its diuretic and analgesic properties. Nevertheless, few studies investigated possible biological effects of extracts. Further, evidence of antioxidant actions encourages the investigation of possible neuroprotective effects in animal models of neurodegenerative diseases. This study investigates the effect of the ethanolic extract (PAS) on mice submitted to a progressive model of Parkinson's disease (PD) induced by reserpine. Male (6-month-old) mice received reserpine (0.1 mg/kg, s.c.), every other day, for 40 days, with or without a concomitant treatment with daily injections of PAS (25 mg/kg, i.p.). Catalepsy, open field, oral movements, and plus-maze discriminative avoidance evaluations were performed across treatment, and immunohistochemistry for tyrosine hydroxylase was conducted at the end. The results showed that PAS treatment delayed the onset of motor impairments and prevented the occurrence of increased catalepsy behavior in the premotor phase. However, PAS administration did not modify reserpine-induced cognitive impairments. Moreover, PAS prevented the decrease in tyrosine hydroxylase immunostaining in the (SNpc) induced by reserpine. Taken together, our results suggested that PAS exerted a neuroprotective effect in a progressive model of PD.
马斯特斯是巴西本土的西番莲属植物。该属植物在美洲大陆的民间医学中因其利尿和止痛特性而闻名。然而，很少有研究调查提取物可能的生物学效应。此外，抗氧化作用的证据促使人们在神经退行性疾病的动物模型中研究其可能的神经保护作用。本研究调查了乙醇提取物（PAS）对接受利血平诱导的帕金森病（PD）渐进模型的小鼠的影响。雄性（6个月大）小鼠每隔一天接受利血平（0.1 mg/kg，皮下注射），持续40天，同时或不伴有每日注射PAS（25 mg/kg，腹腔注射）的伴随治疗。在整个治疗过程中进行僵住症、旷场试验、口腔运动和加迷宫辨别回避评估，并在最后进行酪氨酸羟化酶的免疫组织化学检测。结果表明，PAS治疗延迟了运动障碍的发作，并防止了运动前期僵住症行为的增加。然而，PAS给药并未改变利血平诱导的认知障碍。此外，PAS防止了利血平诱导的黑质致密部（SNpc）酪氨酸羟化酶免疫染色的减少。综上所述，我们的结果表明PAS在PD渐进模型中发挥了神经保护作用。
