Department of Gastric Cancer Surgery, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy, Tianjin, China.
Dig Dis Sci. 2013 Dec;58(12):3494-502. doi: 10.1007/s10620-013-2837-0. Epub 2013 Aug 25.
Although many researchers have concentrated on the mechanism of T cell anergy resulting from over-expression of Indoleamine 2,3-dioxygenase (IDO), it remains unclear what alterations will developed in memory T cells (Tm) under over-expression of IDO.
Immunohistochemical staining for IDO expression in gastric cancer tissues (n=50) was carried out. Tumor-infiltrating memory Tm cells were counted by flow cytometry. The association between IDO expression and the subsets of tumor infiltrating Tm are discussed.
The higher IDO expressions were significantly associated with deeper invasion (P=0.016) and higher frequencies (P=0.038) of lymph node metastasis. The lower tumor-infiltrating CD4+Tm were significantly associated with the advanced clinical stage (P=0.026) and lymph node metastasis (P=0.016). The lower percentages of CD8+Tm were significantly related to undifferentiated histological type (P=0.042) and lymph node metastasis (P=0.037). However, the lower percentage of CD8+Tem was significantly correlated to differentiated histological type (P=0.017), lower frequencies of lymph node metastasis (P=0.014), and earlier clinical stage (P=0.008). The higher IDO expression patients had significantly lower percentages of CD4+Tm (P=0.012) and CD8+Tm (P=0.033). Nevertheless, it was confirmed that the higher level of IDO expression correlated with higher percentages of CD8+Tm cells in univariate and multivariate analysis (P=0.011).
IDO over-expression and Tm in tumor microenvironments were correlated with the disease stage and histological type of gastric cancer. Higher IDO expression was related to the lower percentage of CD4+Tm and CD8+Tm, whereas the higher level of IDO expression related with a higher percentage of CD8+Tem.
虽然许多研究人员专注于由于吲哚胺 2,3-双加氧酶（IDO）过度表达导致 T 细胞失能的机制，但 IDO 过度表达下记忆 T 细胞（Tm）会发生什么变化仍不清楚。
对 50 例胃癌组织中的 IDO 表达进行免疫组织化学染色。通过流式细胞术计数肿瘤浸润性记忆 Tm 细胞。讨论了 IDO 表达与肿瘤浸润性 Tm 亚群之间的关系。
较高的 IDO 表达与更深的浸润（P=0.016）和更高的淋巴结转移频率（P=0.038）显著相关。较低的肿瘤浸润性 CD4+Tm 与较晚的临床分期（P=0.026）和淋巴结转移（P=0.016）显著相关。较低的 CD8+Tm 百分比与未分化组织学类型（P=0.042）和淋巴结转移（P=0.037）显著相关。然而，较低的 CD8+Tem 百分比与分化组织学类型显著相关（P=0.017）、较低的淋巴结转移频率（P=0.014）和较早的临床分期（P=0.008）。IDO 表达较高的患者 CD4+Tm（P=0.012）和 CD8+Tm（P=0.033）的百分比显著降低。然而，在单变量和多变量分析中，均证实 IDO 表达水平越高与 CD8+Tm 细胞的百分比越高相关（P=0.011）。
IDO 过度表达和肿瘤微环境中的 Tm 与胃癌的疾病分期和组织学类型相关。较高的 IDO 表达与 CD4+Tm 和 CD8+Tm 的百分比降低有关，而较高的 IDO 表达水平与 CD8+Tem 的百分比增加有关。
