Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, P.O. 550, Truro, NS, B2N 5E3, Canada.
J Chem Ecol. 2013 Sep;39(9):1169-81. doi: 10.1007/s10886-013-0337-5. Epub 2013 Aug 27.
Blueberry spanworm, Itame argillacearia (Packard), is an important defoliator of lowbush (syn. 'wild') blueberry, Vaccinium angustifolium Aiton, in north-eastern North America. The goal of the present study was to identify the female I. argillacearia sex pheromone, which could be used in traps for monitoring or mass-trapping this pest. Gas chromatography/mass spectrometry (GC/MS) and electroantennogram (EAG) recordings of sex pheromone gland extracts, in combination with chemical synthesis, a Y-tube olfactometer study and field experiments confirmed (2R,3S)-2-ethyl-3-((Z,Z)-tridecadi-2,5-enyl) oxirane (hereafter (Z,Z)-(3R,4S)-3,4-epoxy-6,9-heptadecadiene) and (Z,Z,Z)-3,6,9-heptadecatriene as female-produced sex pheromone components. (Z,Z)-(3R,4S)-3,4-Epoxy-6,9-heptadecadiene elicited a response from male I. argillacearia antennae during EAG recording, and in the Y-tube olfactometer tests males did not discriminate between a live female and (Z,Z)-(3R,4S)-3,4-epoxy-6,9-heptadecadiene. Field-trapping experiments showed that a blend of (Z,Z)-(3R,4S)-3,4-epoxy-6,9-heptadecadiene and (Z,Z,Z)-3,6,9-heptadecatriene was more attractive to male moths than (Z,Z)-(3R,4S)-3,4-epoxy-6,9-heptadecadiene alone.
蓝莓卷叶蛾，Itame argillacearia (Packard)，是北美东北部低地（又称‘野生’）蓝莓 Vaccinium angustifolium Aiton 的一种重要食叶害虫。本研究的目的是鉴定蓝莓卷叶蛾的雌性信息素，以便用于监测或大量诱捕这种害虫的诱捕器中。性信息素腺体提取物的气相色谱/质谱联用 (GC/MS) 和触角电位 (EAG) 记录，结合化学合成、Y 型嗅觉仪研究和田间试验证实了 (2R,3S)-2-乙基-3-((Z,Z)-十三碳-2,5-二烯基)环氧乙烷（以下简称 (Z,Z)-(3R,4S)-3,4-环氧-6,9-十七碳二烯）和 (Z,Z,Z)-3,6,9-十七碳三烯是雌性产生的性信息素成分。(Z,Z)-(3R,4S)-3,4-环氧-6,9-十七碳二烯在 EAG 记录期间引起雄性蓝莓卷叶蛾触角的反应，并且在 Y 型嗅觉仪测试中，雄性对活体雌性和 (Z,Z)-(3R,4S)-3,4-环氧-6,9-十七碳二烯之间没有辨别能力。田间诱捕实验表明，(Z,Z)-(3R,4S)-3,4-环氧-6,9-十七碳二烯和 (Z,Z,Z)-3,6,9-十七碳三烯的混合物比单独使用 (Z,Z)-(3R,4S)-3,4-环氧-6,9-十七碳二烯对雄性蛾更具吸引力。
