Division of Biology and Genetics, Department of Molecular and Translational Medicine and National Institute of Neuroscience, University of Brescia, Viale Europa 11, 25123, Brescia, Italy.
J Mol Neurosci. 2013 Nov;51(3):903-10. doi: 10.1007/s12031-013-0098-1. Epub 2013 Aug 27.
We have previously observed changes in the RNA editing of AMPA receptors after acute spinal cord injury (SCI); this implies that post-transcriptional modifications are capable of affecting the physiological properties of glutamate receptor channels and related signal transduction in this neurodegenerative condition. Here, we report that the editing of the ionotropic KAR is markedly decreased at both GluK1 and GluK2 Q/R sites in the epicenter of the lesion and with distinct magnitude and kinetics also in the caudal and rostral portions of the injured cord. These effects are persistent, being observed as late as 30 days after lesioning. In addition, also the I/V and Y/C sites of GluK2 were severely affected after SCI. These findings add novel information to the relevance of editing of glutamate receptors following acute SCI, thus expanding the recently emerged role of post-transcriptional mechanisms under these experimental conditions.
我们之前观察到急性脊髓损伤 (SCI) 后 AMPA 受体的 RNA 编辑发生变化；这意味着转录后修饰能够影响谷氨酸受体通道的生理特性和这种神经退行性疾病中的相关信号转导。在这里，我们报告说，在损伤中心，KAR 的离子型 GluK1 和 GluK2 Q/R 位点的编辑明显减少，并且在损伤脊髓的尾部和头部也具有明显的幅度和动力学。这些影响是持久的，在损伤后 30 天仍可观察到。此外，SCI 后 GluK2 的 I/V 和 Y/C 位点也受到严重影响。这些发现为急性 SCI 后谷氨酸受体编辑的相关性提供了新的信息，从而扩展了最近在这些实验条件下转录后机制的作用。
