Cardiothoracic Surgery Section, The Third Affiliated Hospital of Kunming Medical University (Yunnan Province Cancer Hospital), Lung Cancer Research Center, Yunnan Institute of Oncology, 519 Kun-Zhou Road, Kunming, 650118, Yunnan, China.
Cell Biochem Biophys. 2013;67(3):1501-5. doi: 10.1007/s12013-013-9652-2.
To study the influence of brominated furanones on the formation of Staphylococcus aureus (SA) biofilm on PVC thus providing new avenues of research on the surface modification of materials and clinical treatment of biomaterial-centered infection. Three brominated furanones (furanone-1, furanone-2, and furanone-3) were coated on the surface of PVC material. Both the modified PVC materials and SA were co-cultivated together. To assess the thickness of bacterial biofilm and bacterium colony unit area on PVC materials, confocal laser scanning microscopy and scanning electron microscopy (SEM) were used to observe the surface structure of SA biofilm formation. All treatments were compared with the control group which was not coated with furanones. PVC materials coated with furanone-1 had an increase in bacterial biofilm as well as SA colony area when compared with control. However, there was no significant difference between treating with furanone-1 and furanone-3 (P > 0.05). The impact of different brominated furanones on SA biofilm formation on the surface of PVC materials is different, furanone-1 can promote the SA biofilm formation on the surface of PVC material.
研究溴代呋喃酮对金黄色葡萄球菌（SA）在聚氯乙烯（PVC）表面形成生物膜的影响，从而为材料表面改性和生物材料相关感染的临床治疗提供新的研究途径。将三种溴代呋喃酮（呋喃酮-1、呋喃酮-2 和呋喃酮-3）涂覆在 PVC 材料表面。将改性后的 PVC 材料和 SA 一起共同培养。为了评估 PVC 材料上细菌生物膜的厚度和细菌菌落单位面积，使用共聚焦激光扫描显微镜和扫描电子显微镜（SEM）观察 SA 生物膜形成的表面结构。所有处理均与未涂覆呋喃酮的对照组进行比较。与对照组相比，涂覆呋喃酮-1 的 PVC 材料的细菌生物膜和 SA 菌落面积均增加。然而，呋喃酮-1 和呋喃酮-3 之间的处理没有显著差异（P > 0.05）。不同溴代呋喃酮对 PVC 材料表面 SA 生物膜形成的影响不同，呋喃酮-1 可以促进 SA 在 PVC 材料表面形成生物膜。
