Ye Lianhua, Xu Geng, Huang Yunchao, Zhou Youquan, Zhao Guangqiang, Lei Yujie
Department of Thoracic Surgery, Lung Cancer Research Centre of the Cancer Institute of Yunnan Province, Kunming Yunnan, 650118, P R China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2010 Jul;24(7):871-4.
To study the influence of brominated furanones on the biofilm formation of Escherichia coli on the polyvinyl chloride (PVC) material, and to provide new ideas for the research of surface modification of materials and clinical treatment of biomaterial centered infection.
Three brominated furanones with representative chemical structure were chosen and coated on the surface modification of PVC materials, respectively [furanone 1: 3, 4-dibromo-5-hydroxy-furanone; furanone 2: 4-bromo-5-(4-methoxyphenyl)-3-(methylamino)-furanone; furanone 3: 3, 4-dibromo-5, 5-bis (4-methylphenyl)-2 (5H)-furanone]. All the modificated PVC materials and Escherichia coli were co-cultivated. The PVC material soaked with 75% ethanol for 5 minutes and Escherichia coli were co-cultivated together as the control group. The thickness of bacterial community and bacterial community quantity in the unit area on PVC materials were measured by confocal laser scanning microscope (CLSM), and the surface structure of biofilm formation was observed by scanning electron microscope (SEM).
The CLSM showed that the thickness of bacterial community and the bacterial community quantity in the unit area of PVC materials was significantly less (P < 0.05) in furanone 3 group than in control group, but no significant difference (P > 0.05) was found between furanone 1, furanone 2 groups and control group. SEM showed that the quantity of bacterial community in the unit area of PVC materials surface in furanone 3 group was fewer than that in control group at 6 hours; the biofilm structure on PVC materials surface formed at 18 hours in control group, furanone 1 group, and furanone 2 group, but there was no mature biofilm structure on PVC materials surface in furanone 3 group at 18 hours.
The impact of different brominated furanones on Escherichia coli biofilm formation on the surface of PVC materials is different, 3, 4-dibromo-5, 5-bis (4-methylphenyl)-2 (5H)-furanone can inhibit Escherichia coli biofilm formation on the surface of PVC material. Polyvinyl chloride
研究溴代呋喃酮对大肠杆菌在聚氯乙烯（PVC）材料上生物膜形成的影响，为材料表面改性及以生物材料为中心的感染临床治疗研究提供新思路。
选取三种具有代表性化学结构的溴代呋喃酮，分别对PVC材料进行表面改性处理[呋喃酮1：3,4 - 二溴 - 5 - 羟基 - 呋喃酮；呋喃酮2：4 - 溴 - 5 -（4 - 甲氧基苯基）- 3 -（甲氨基）- 呋喃酮；呋喃酮3：3,4 - 二溴 - 5,5 - 双（4 - 甲基苯基）- 2（5H）- 呋喃酮]。将所有改性后的PVC材料与大肠杆菌共同培养。将用75%乙醇浸泡5分钟的PVC材料与大肠杆菌共同培养作为对照组。采用共聚焦激光扫描显微镜（CLSM）测量PVC材料上单位面积的细菌群落厚度和细菌群落数量，并用扫描电子显微镜（SEM）观察生物膜形成的表面结构。
CLSM显示，呋喃酮3组PVC材料单位面积的细菌群落厚度和细菌群落数量均显著低于对照组（P < 0.05），但呋喃酮1组、呋喃酮2组与对照组之间无显著差异（P > 0.05）。SEM显示，呋喃酮3组在6小时时PVC材料表面单位面积的细菌群落数量少于对照组；对照组、呋喃酮1组和呋喃酮2组在18小时时PVC材料表面形成生物膜结构，但呋喃酮3组在18小时时PVC材料表面无成熟生物膜结构。
不同溴代呋喃酮对PVC材料表面大肠杆菌生物膜形成的影响不同，3,4 - 二溴 - 5,5 - 双（4 - 甲基苯基）- 2（5H）- 呋喃酮可抑制大肠杆菌在PVC材料表面形成生物膜。聚氯乙烯
