Alcoforado G A, Kristoffersen T, Johannessen A C, Nilsen R
Department of Periodontology, Escola Superior de Medicina Dentária, Lisboa, Portugal.
J Clin Periodontol. 1990 Jul;17(6):335-40. doi: 10.1111/j.1600-051x.1990.tb00027.x.
Gingival biopsies were obtained from 23 children, aged 5-11 years (8.6 +/- 1.8 years). Specimens were taken from areas of the gingiva adjacent to the teeth which were to be extracted because of caries or its sequelae and which clinically had a gingival index score of at least 1. Staining for alpha-naphthyl acetate esterase with unspecific esterase at pH 5.8 (ANAE) permitted identification of T lymphocytes, monocytes/macrophages, plasma cells and non-reactive (ANAE-negative) cells. Cells which tentatively were identified as "natural killer" (NK) cells were also observed. Differential cell counting was performed for 10 specimens, selected on the basis of the presence of a well-defined inflammatory infiltrate, clear morphology throughout and good ANAE staining. Cell counts confirmed earlier studies showing that lymphocytes predominate in the inflammatory infiltrates in childrens' gingivitis. T lymphocytes dominated particularly in the periphery of the most densely infiltrated areas. Relatively few plasma cells were seen. It was concluded that T lymphocytes dominate in the inflammatory infiltrate in childrens' gingivitis.
从23名5至11岁（平均年龄8.6±1.8岁）的儿童身上获取牙龈活检样本。样本取自因龋齿或其后遗症而即将拔除的牙齿附近的牙龈区域，这些区域临床牙龈指数评分至少为1。在pH 5.8条件下用非特异性酯酶对α-萘乙酸酯酶进行染色（ANAE），可识别T淋巴细胞、单核细胞/巨噬细胞、浆细胞和非反应性（ANAE阴性）细胞。还观察到了初步鉴定为“自然杀伤”（NK）细胞的细胞。对10个样本进行了细胞分类计数，这些样本是根据明确的炎症浸润、整体清晰的形态学以及良好的ANAE染色来选择的。细胞计数证实了早期研究结果，即淋巴细胞在儿童牙龈炎的炎症浸润中占主导地位。T淋巴细胞尤其在浸润最密集区域的周边占主导。可见相对较少的浆细胞。得出的结论是，T淋巴细胞在儿童牙龈炎的炎症浸润中占主导。
