Matus A, Delhaye-Bouchaud N, Mariani J
Friedrich Miescher-Institut, Basel, Switzerland.
J Comp Neurol. 1990 Jul 15;297(3):435-40. doi: 10.1002/cne.902970308.
We have studied the distribution of microtubule-associated protein 2 (MAP2) in the Purkinje cell dendrites of rats whose cerebella were exposed to X-irradiation during the second postnatal week. The Purkinje cells of such animals have abnormally elongated apical primary processes that branch in the other molecular layer rather than close to the cell body as in normal tissue. The results show that in these distorted dendrites the MAP2 distribution is "shifted" distally relative to the normal pattern, in which MAP2 is distributed evenly throughout the dendritic tree. Tubulin and other microtubule-associated proteins, such as MAP1, are not affected and remain evenly distributed throughout the dendritic tree despite the anatomical distortion. We conclude that the distribution of MAP2 in Purkinje cells is not determined solely by its binding to tubulin. Other factors must be involved and these appear to be related to dendritic morphology and possibly to branching.
我们研究了在出生后第二周小脑接受X射线照射的大鼠浦肯野细胞树突中微管相关蛋白2（MAP2）的分布情况。这类动物的浦肯野细胞具有异常伸长的顶端初级突起，这些突起在另一个分子层分支，而不是像正常组织那样在靠近细胞体的位置分支。结果表明，在这些扭曲的树突中，MAP2的分布相对于正常模式“向远端偏移”，在正常模式中MAP2均匀分布于整个树突树。微管蛋白和其他微管相关蛋白，如MAP1，不受影响，尽管存在解剖学上的扭曲，它们仍均匀分布于整个树突树。我们得出结论，MAP2在浦肯野细胞中的分布并非仅由其与微管蛋白的结合所决定。必然涉及其他因素，这些因素似乎与树突形态有关，可能还与分支有关。
