Bernhardt R, Matus A
J Comp Neurol. 1984 Jun 20;226(2):203-21. doi: 10.1002/cne.902260205.
A specific antiserum was used to ascertain the distribution of microtubule-associated protein 2 (MAP2) in the rat brain at the light and electron microscope levels. Light microscopy showed MAP2 to be present only in neurons, and only in the dendrites and the perikaryon of each cell. This same polarized distribution pattern was found in the Purkinje, Golgi, basket, stellate, and granule cells of the cerebellum, and also in neurons of the hippocampus, the olfactory bulb, and the midbrain. While labelling of the dendritic arborization was extensive and intense, MAP2 density tended to decrease in the proximal dendritic trunk. Particularly in large neurons (e.g., Purkinje, Golgi, and pyramidal cells), staining was reproducibly weaker in the cell body than in the main dendrites. Dendritic contours generally appeared smooth, without any evidence of staining of dendritic spines. An electron microscope examination of the cerebellum confirmed the presence of MAP2 reactivity in neurons and its absence from axons and non-neuronal cells. MAP2 in dendrites was associated with microtubules, while MAP2 in neuronal perikarya was associated with polyribosomes. There was no evidence of specific staining in dendritic spines and in postsynaptic densities. MAP2 is a novel dendritic marker and labels part of a specific dendritic cytoskeleton, different from that in axons and non-neuronal cells.
使用一种特异性抗血清在光学显微镜和电子显微镜水平上确定大鼠脑中微管相关蛋白2（MAP2）的分布。光学显微镜显示MAP2仅存在于神经元中，且仅存在于每个细胞的树突和胞体中。在小脑的浦肯野细胞、高尔基细胞、篮状细胞、星状细胞和颗粒细胞中，以及海马、嗅球和中脑的神经元中都发现了相同的极化分布模式。虽然树突分支的标记广泛且强烈，但MAP2密度在近端树突干中趋于降低。特别是在大型神经元（如浦肯野细胞、高尔基细胞和锥体细胞）中，细胞体中的染色始终比主要树突中的染色弱。树突轮廓通常看起来很光滑，没有任何树突棘染色的迹象。对小脑的电子显微镜检查证实了神经元中存在MAP2反应性，而轴突和非神经元细胞中不存在。树突中的MAP2与微管相关，而神经元胞体中的MAP2与多聚核糖体相关。在树突棘和突触后致密物中没有特异性染色的证据。MAP2是一种新型的树突标记物，标记特定树突细胞骨架的一部分，不同于轴突和非神经元细胞中的细胞骨架。
