Center for Digestive Health, 1817 North Mills Avenue, Orlando, FL, 32803, USA,
Dig Dis Sci. 2013 Dec;58(12):3422-8. doi: 10.1007/s10620-013-2846-z. Epub 2013 Aug 28.
Non-variceal upper gastrointestinal (UGI) bleeding is a common condition that requires prompt lifesaving therapy and traditional endoscopic treatments require high technical proficiency to perform.
This study was conducted to identify any local or systemic histopathologic effects of a hemostatic powder in a porcine model of active, severe, non-variceal UGI hemorrhage.
This prospective, non-blinded animal study was performed in accordance with Good Laboratory Practice and Animal Care and Use Guidelines. Six animals underwent gastrotomy and creation of a looped vascular bundle, which was placed into the stomach lumen. The transplanted vascular bundle was punctured with an endoscopic needle-knife to create Forrest grade Ia or Ib bleeding. The hemostatic powder was then applied until hemostasis was achieved.
Initial hemostasis was achieved in all animals. Results of pre- and post-treatment coagulation studies were similar. All animals survived at least 9 days post-procedure. The hemostatic powder was not found in any local, regional, or systemic tissues. Gross and histologic analysis of systemic organs showed no infarct, particulate, or embolic effects. No gross or microscopic necropsy findings were treatment-related.
The hemostatic powder achieved initial hemostasis (even in animals with spurting arterial bleeding) with no bowel obstruction or unintended luminal effects, no local or regional particulate effects, no systemic embolic effects, and no systemic coagulopathic effects.
非静脉曲张性上消化道（UGI）出血是一种常见病症，需要立即进行救生治疗，而传统的内镜治疗需要高超的技术才能进行。
本研究旨在确定一种止血粉在猪活动性、严重非静脉曲张性 UGI 出血模型中的局部或全身组织病理学效应。
这项前瞻性、非盲法动物研究符合良好实验室规范和动物护理使用指南。六只动物接受胃切开术和环型血管束的创建，将其放入胃腔中。用内镜针刀刺穿移植的血管束，造成 Forrest 分级 Ia 或 Ib 出血。然后应用止血粉直至止血。
所有动物均实现了初始止血。凝血研究的治疗前后结果相似。所有动物在手术后至少 9 天都存活下来。止血粉未在任何局部、区域或全身组织中发现。对全身器官的大体和组织学分析未显示梗死、颗粒或栓塞效应。大体和显微镜尸检结果与治疗无关。
止血粉实现了初始止血（即使在动脉喷血的动物中也是如此），没有肠阻塞或意外的腔道效应，没有局部或区域颗粒效应，没有全身栓塞效应，也没有全身凝血功能障碍效应。
