Moore C M
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
J Forensic Sci Soc. 1990 May-Jun;30(3):123-9. doi: 10.1016/s0015-7368(90)73322-1.
The extraction of basic drugs from the urine of racing greyhounds has been carried out for many years using solvent extraction. This paper describes an extraction method that utilises a cation exchange column. Fourteen basic drugs were introduced onto the column in their ionised form, washed with organic and aqueous solvents and eluted with basic methanol. Three drugs, cyclizine, procaine and quinine were determined in authentic samples from racing greyhounds. A comparison of the solid-phase method with liquid-liquid extraction showed an increase in the efficiency of extraction and a reduction in both the time and cost incurred. The method requires a minimal volume of sample and solvent, little glassware and is easily automated.
多年来一直采用溶剂萃取法从赛犬尿液中提取碱性药物。本文描述了一种利用阳离子交换柱的萃取方法。14种碱性药物以离子化形式被引入柱中，先用有机溶剂和水性溶剂冲洗，再用碱性甲醇洗脱。在赛犬的真实样本中测定了3种药物，即赛克利嗪、普鲁卡因和奎宁。将固相萃取法与液液萃取法进行比较，结果表明萃取效率提高，同时时间和成本都有所降低。该方法所需样本和溶剂体积最小，玻璃器皿用量少，且易于自动化操作。
