Zhou Jian, Ge Bao-Feng, Chen Ke-Ming, Ma Xiao-Ni, Cheng Kui, Guo Xiao-Yu, Lü Xiang
Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
Yao Xue Xue Bao. 2013 Jun;48(6):960-4.
This study is to investigate effects of genistein on rat femoral bone metabolic in vitro. Rat femoral tissues was isolated and randomly divided into two groups including control group and genistein (1 x 10(-5) mol x(-1)) group. Determinations of alkaline phosphatase (ALP) activity, calcium content and osteoprotegerin (OPG), type I-collagen (Collagen-I), RANKL, Runx-2 and bone morphogenetic protein (BMP-2) mRNA expression were done by real-time PCR. The results showed that 1 x 10(-5) mol x L(-1) genistein could increase the activity of ALP and contents of Ca, regulate bone metabolism activity of OPG, RANKL, BMP-2, Collagen-I and Runx-2 mRNA expression level. Genistein can significantly modulate bone metabolism related gene expression level of rat femoral tissue in vitro, and can increase calcium content and the activity of ALP.
本研究旨在体外研究染料木黄酮对大鼠股骨骨代谢的影响。分离大鼠股骨组织并随机分为两组，包括对照组和染料木黄酮（1×10⁻⁵mol·L⁻¹）组。通过实时聚合酶链反应测定碱性磷酸酶（ALP）活性、钙含量以及骨保护素（OPG）、I型胶原蛋白（Collagen-I）、核因子κB受体活化因子配体（RANKL）、 runt相关转录因子2（Runx-2）和骨形态发生蛋白（BMP-2）mRNA表达。结果表明，1×10⁻⁵mol·L⁻¹染料木黄酮可增加ALP活性和钙含量，调节OPG、RANKL、BMP-2、Collagen-I和Runx-2 mRNA表达水平的骨代谢活性。染料木黄酮可在体外显著调节大鼠股骨组织骨代谢相关基因表达水平，并可增加钙含量和ALP活性。
