Babich H, Borenfreund E
Rockefeller University, Laboratory Animal Research Center, New York, NY 10021.
J Pharm Sci. 1990 Jul;79(7):592-4. doi: 10.1002/jps.2600790709.
The acute cytotoxicity of butylated hydroxytoluene and butylated hydroxyanisole to cultured human dermal fibroblasts, keratinocytes, melanocytes, and melanoma tumor cells was determined with the neutral red assay. For all cell types, butylated hydroxytoluene proved to be more cytotoxic than butylated hydroxyanisole. The neutral red assay is a rapid, economical, semiautomated assay that can be used with a variety of cell types in culture to provide quantitated data that can be used to rank test agents according to their potencies.
采用中性红试验测定了丁基化羟基甲苯和丁基化羟基茴香醚对培养的人皮肤成纤维细胞、角质形成细胞、黑素细胞和黑色素瘤肿瘤细胞的急性细胞毒性。对于所有细胞类型，丁基化羟基甲苯的细胞毒性均高于丁基化羟基茴香醚。中性红试验是一种快速、经济的半自动化试验，可用于培养中的多种细胞类型，以提供定量数据，用于根据效力对受试物进行排名。
