不同培养条件下真菌弗氏节丛孢菌和奇异单顶孢菌的菌丝体大量生产
Mycelial mass production of fungi Duddingtonia flagrans and Monacrosporium thaumasium under different culture conditions.
作者信息
da Silva Manoel Eduardo, de Araújo Jackson Victor, Braga Fabio Ribeiro, Borges Luana Alcântara, Soares Filippe Elias F, Lima Walter dos Santos, Guimarães Marcos Pezzi
机构信息
Departamento de Veterinária, UFV, Viçosa, MG, Brasil.
出版信息
BMC Res Notes. 2013 Aug 29;6:340. doi: 10.1186/1756-0500-6-340.
BACKGROUND
Duddingtonia flagrans and Monacrosporium thaumasium are promising fungus species in veterinary biological control of gastrointestinal nematodes because of their production capacity of fungal structures (conidia and/or chlamydospores), growth efficiency in laboratory solid media and especially their predatory capacity. However, their large-scale production remains a challenge. This work aimed at evaluating the mycelial mass production of D. flagrans (AC001 and CG722) and M. thaumasium (NF34A) nematophagous fungi under different culture conditions.
RESULTS
The results did not present significant differences (p > 0.05) in mycelia mass production between the isolates cultured under pH 4.0. Furthermore, after 168 hrs., the isolate CG722 presented a lower production of mycelial mass in medium CM (corn meal) (p < 0.05).
CONCLUSION
We therefore concluded the use of culture media SD (soy dextrose) and CG (corn grits) at pH values between 6.0 and 7.0 is suitable for high mycelial mass production of D. flagrans and M. thaumasium.
背景
由于能够产生真菌结构（分生孢子和/或厚垣孢子）、在实验室固体培养基中的生长效率，特别是其捕食能力，达丁顿孢子虫和奇异单顶孢是胃肠道线虫兽医生物防治中很有前景的真菌物种。然而，它们的大规模生产仍然是一个挑战。这项工作旨在评估在不同培养条件下达丁顿孢子虫（AC001和CG722）和奇异单顶孢（NF34A）这两种食线虫真菌的菌丝体产量。
结果
在pH 4.0条件下培养的分离株之间，菌丝体产量没有显著差异（p > 0.05）。此外，168小时后，分离株CG722在CM（玉米粉）培养基中的菌丝体产量较低（p < 0.05）。
结论
因此，我们得出结论，使用pH值在6.0至7.0之间的SD（大豆葡萄糖）和CG（玉米粗粉）培养基适合于高效生产达丁顿孢子虫和奇异单顶孢的菌丝体。
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