College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China.
Food Chem Toxicol. 2013 Dec;62:432-5. doi: 10.1016/j.fct.2013.08.041. Epub 2013 Aug 25.
5-Hydroxy-6,7,3',4'-tetramethoxyflavone (HTF), limonexic acid (LA) are two compounds isolated from the flowers of Citrus aurantium L. var. amara Engl with various biological activities. This study was designed to investigate their protective effects against carbon tetrachloride (CCl4)-induced hepatocyte injury, using human hepatic cell line HL-7702 to determine the cell cytotoxicity, cell viability, levels of hepatic marker enzymes, malondialdehyde (MDA). Results showed that pretreatment with HTF, LA could significantly reverse CCl4-induced HL-7702 cell viability decrease, LA displayed a higher activity. HTF, LA also showed their capability of decreasing the CCl4-induced leakage of lactate dehydrogenase (LDH), aspartate aminotransferase (AST), inhibiting the lipid peroxidation, HTF showed more significant activity. Given that HTF, LA were not toxic, it is concluded that HTF, LA could effectively protect hepatocyte against CCl4-induced injury.
5-羟基-6,7,3',4'-四甲氧基黄酮（HTF）和柠檬苦素酸（LA）是从芸香科柑橘属植物酸橙的花中分离得到的两种具有多种生物活性的化合物。本研究旨在探讨它们对四氯化碳（CCl4）诱导的肝细胞损伤的保护作用，用人肝细胞系 HL-7702 测定细胞毒性、细胞活力、肝标志物酶和丙二醛（MDA）水平。结果表明，HTF、LA 预处理可显著逆转 CCl4 诱导的 HL-7702 细胞活力下降，LA 显示出更高的活性。HTF、LA 还显示出降低 CCl4 诱导的乳酸脱氢酶（LDH）、天冬氨酸转氨酶（AST）漏出的能力，抑制脂质过氧化，HTF 表现出更显著的活性。鉴于 HTF、LA 没有毒性，可以得出结论，HTF、LA 可以有效保护肝细胞免受 CCl4 诱导的损伤。
