Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
J Pharm Pharmacol. 2009 Nov;61(11):1511-20. doi: 10.1211/jpp/61.11.0011.
Rubus sanctus Schreb., known from the Bible as 'holy thorn bush', grows wild in Egypt. Rubus sanctus aqueous alcoholic extract (RE) contains a complicated phenolic mixture (ellagitanins, flavonoids and caffeic acid derivatives). In this study, the phytochemical investigation of the plant was re-evaluated. Herein, we report on the isolation and identification of three galloylated flavonoids, namely kaempferol-3-O-(6''-O-galloyl)-(4)C(1)-beta-d-galactopyranoside, quercetin-3-O-(6''-O-galloyl)-(4)C(1)-beta-d-galactopyranoside and myricetin-3-O-(6''-O-galloyl)-(4)C(1)-beta-d-galactopyranoside for the first time from the Rubus genus. We further aimed at evaluating the potential protective effects of RE against carbon tetrachloride (CCl(4))-induced toxicity in isolated rat hepatocytes.
Based on an initial concentration-response experiment, a concentration of 100 mug/ml was selected to investigate the hepatoprotective activity of RE.
Pretreatment with RE afforded protection as indicated by counteracting CCl(4)-induced cell death, and reduced glutathione depletion. In addition, RE ameliorated CCl(4)-induced enzyme leakage by 40% for lactate dehydrogenase, 30% for alanine aminotransferase and 20% for aspartate aminotransferase as compared with CCl(4)-treated cells. Moreover, RE counteracted CCl(4)-induced lipid peroxidation and inhibited spontaneous lipid peroxidation in the control group.
In conclusion, RE protects against CCl(4)-induced toxicity in isolated rat hepatocytes.
圣经中提到的“圣荆棘灌木”Rubus sanctus Schreb. 在埃及野生生长。Rubus sanctus 水醇提取物（RE）含有复杂的酚类混合物（鞣花单宁、类黄酮和咖啡酸衍生物）。在这项研究中，重新评估了该植物的植物化学研究。本文报告了三种酰化黄酮的分离和鉴定，即山奈酚-3-O-(6”-O- 酰基)-(4)C(1)-β-d-半乳糖吡喃糖苷、槲皮素-3-O-(6”-O- 酰基)-(4)C(1)-β-d-半乳糖吡喃糖苷和杨梅素-3-O-(6”-O- 酰基)-(4)C(1)-β-d-半乳糖吡喃糖苷，这是首次从悬钩子属植物中分离得到。我们进一步旨在评估 RE 对四氯化碳（CCl(4)）诱导的离体大鼠肝细胞毒性的潜在保护作用。
基于初始浓度-反应实验，选择 100μg/ml 的浓度来研究 RE 的肝保护活性。
RE 的预处理提供了保护作用，表现在对抗 CCl(4)诱导的细胞死亡和还原型谷胱甘肽耗竭。此外，与 CCl(4)处理的细胞相比，RE 使乳酸脱氢酶、丙氨酸转氨酶和天冬氨酸转氨酶的 CCl(4)诱导的酶漏出分别减少了 40%、30%和 20%。此外，RE 对抗 CCl(4)诱导的脂质过氧化作用，并抑制对照组的自发性脂质过氧化作用。
总之，RE 可防止 CCl(4)诱导的离体大鼠肝细胞毒性。
