Fukami Harukazu, Yano Yoshihisa, Iwashita Takashi
Department of Bioscience and Biotechnology, Faculty of Bioenvironmental Science, Kyoto Gakuen University, Kyoto, Japan.
J Oleo Sci. 2013;62(8):623-9. doi: 10.5650/jos.62.623.
5,7,3',4'-Tetrahydroxyflav-2-en-3-ol 3-O-β-D-glucoside was isolated from the seed coats of immature black soybeans (Glycine max (L.) Merr.). This compound is a reduced form of cyanidin 3-O-β-D-glucoside (cyanidin 3-G) which was obtained by reaction with hydrochloric acid. The molecule has reducing activity for a tetrazolium derivative (WST-1) in the presence of 1-methoxy-5-methylphenazinium methylsulfate (1-methoxy PMS) in a similar manner to NADH. The seed coats of immature black soybeans also contain epicatechin as a major constituent, while cyanidin 3-G and procyanidin B2 are present at lower concentrations. Immature brown soybeans did not contain 5,7,3',4'-tetrahydroxyflav-2-en-3-ol 3-O-β-D-glucoside, but did contain both epicatechin and procyanidin B2. Immature yellow soybeans contained none of them.
5,7,3',4'-四羟基黄烷-2-烯-3-醇3-O-β-D-葡萄糖苷是从未成熟黑豆（Glycine max (L.) Merr.）的种皮中分离得到的。该化合物是花青素3-O-β-D-葡萄糖苷（花青素3-G）与盐酸反应后的还原形式。在1-甲氧基-5-甲基吩嗪硫酸甲酯（1-甲氧基PMS）存在的情况下，该分子对四唑盐衍生物（WST-1）具有还原活性，其方式与NADH类似。未成熟黑豆的种皮还以表儿茶素作为主要成分，而花青素3-G和原花青素B2的含量较低。未成熟的棕色大豆不含5,7,3',4'-四羟基黄烷-2-烯-3-醇3-O-β-D-葡萄糖苷，但含有表儿茶素和原花青素B2。未成熟的黄色大豆则均不含有这些成分。
