Katsuzaki Hirotaka, Hibasami Hiroshige, Ohwaki Shinji, Ishikawa Kengo, Imai Kunio, Date Kumiko, Kimura Yukinobu, Komiya Takashi
Faculty of Bioresources, Mie University, Mie 514-0001, Japan.
Oncol Rep. 2003 Mar-Apr;10(2):297-300.
Cyanidin 3-O-beta-D-glucoside (CG) was purified from black bean seed coat and other anthocyanins were prepared from red grape skin. These anthocyanins were identified by Mass, and 1H- and 13C-NMR. The effects of four anthocyanins on cell viability in human leukemia Molt 4B cells were investigated. The anthocyanins displayed strong growth inhibitory effects against human leukemia Molt 4B cells. Morphological changes showing apoptotic bodies were observed in the Molt 4B cells treated with these anthocyanins. The fragmentations by these anthocyanins of DNA to oligonucleosomal-sized fragments, that is a characteristic of apoptosis, were observed to be concentration-dependent. N-acetyl-L-cysteine, an antioxidant, suppressed the fragmentation of DNA by these anthocyanins. These findings suggest that growth inhibition of Molt 4B cells by these anthocyanins result from the induction of apoptosis by these compounds and that active oxygen is involved in the induction of apoptosis in the Molt 4B cells.
矢车菊素3 - O - β - D - 葡萄糖苷（CG）从黑豆种皮中纯化得到，其他花青素从红葡萄皮中制备。这些花青素通过质谱、1H - 和13C - NMR进行鉴定。研究了四种花青素对人白血病Molt 4B细胞活力的影响。这些花青素对人白血病Molt 4B细胞显示出强烈的生长抑制作用。在用这些花青素处理的Molt 4B细胞中观察到显示凋亡小体的形态变化。观察到这些花青素使DNA断裂为寡核小体大小的片段，这是凋亡的一个特征，且呈浓度依赖性。抗氧化剂N - 乙酰 - L - 半胱氨酸抑制了这些花青素引起的DNA断裂。这些发现表明，这些花青素对Molt 4B细胞的生长抑制是由这些化合物诱导凋亡所致，并且活性氧参与了Molt 4B细胞凋亡的诱导。
