University of Tartu, Institute of Technology, Tartu, Estonia.
J Virol. 2013 Nov;87(22):12051-68. doi: 10.1128/JVI.01798-13. Epub 2013 Aug 28.
We describe the extensive and progressive oligomerization of human papillomavirus (HPV) genomes after transfection into the U2OS cell line. The HPV genomic oligomers are extrachromosomal concatemeric molecules containing the viral genome in a head-to-tail orientation. The process of oligomerization does not depend on the topology of the input DNA, and it does not require any other viral factors besides replication proteins E1 and E2. We provide evidence that oligomerization of the HPV18 and HPV11 genomes involves homologous recombination. We also demonstrate oligomerization of the HPV18 and HPV11 genomes in SiHa, HeLa, and C-33 A cell lines and provide examples of oligomeric HPV genomes in clinical samples obtained from HPV-infected patients.
我们描述了人乳头瘤病毒（HPV）基因组在转染 U2OS 细胞系后的广泛和渐进的寡聚化。HPV 基因组寡聚物是含有病毒基因组的顺式串联的染色体外连接分子，以头对头的方向排列。寡聚化过程不依赖于输入 DNA 的拓扑结构，除了复制蛋白 E1 和 E2 之外，它不需要任何其他病毒因子。我们提供的证据表明，HPV18 和 HPV11 基因组的寡聚化涉及同源重组。我们还证明了 HPV18 和 HPV11 基因组在 SiHa、HeLa 和 C-33A 细胞系中的寡聚化，并提供了从 HPV 感染患者获得的临床样本中寡聚 HPV 基因组的实例。
