Swapna T S, Binitha M, Manju T S
Department of Botany, SN College, Cherthala, 688 582 Kerala, India.
Appl Biochem Biotechnol. 2004 Jul-Sep;118(1-3):233-41. doi: 10.1385/abab:118:1-3:233.
Kaempferia galanga is an important medicinal plant that is facing threat of extinction owing to indiscriminate and unsustainable harvesting in the wild. Conventional breeding is difficult in this plant, and in vitro multiplication is important to conservation and propagation. Leaf and rhizome explants of Kaempferia were aseptically cultured on MS medium with various combinations of indole-3-acetic acid (IAA), benzyl amino purine (BAP), napthalene acetic acid (NAA), 2-4-dichlorophenoxy acetic acid (2,4-D) and kinetin at concentrations ranging from 0.5 to 2.5 mg/L. High-frequency organogenesis and multiple shoot regeneration was induced from rhizome explants on MS medium supplemented with 0.5 mg/L of IAA and 2.5 mg/L of BAP. Rooting was induced in MS medium with 0.5 mg/L of IAA and 2 mg/L of BAP.
山柰是一种重要的药用植物，由于在野外被滥采滥伐，正面临灭绝的威胁。这种植物进行传统育种很困难，因此离体繁殖对于其保护和繁殖至关重要。将山柰的叶片和根茎外植体在添加了浓度为0.5至2.5毫克/升的吲哚-3-乙酸（IAA）、苄氨基嘌呤（BAP）、萘乙酸（NAA）、2,4-二氯苯氧乙酸（2,4-D）和激动素的各种组合的MS培养基上进行无菌培养。在添加了0.5毫克/升IAA和2.5毫克/升BAP的MS培养基上，根茎外植体诱导出了高频器官发生和多芽再生。在含有0.5毫克/升IAA和2毫克/升BAP的MS培养基上诱导生根。
