Petru Poni Institute of Macromolecular Chemistry, Aleea Gr. Ghica Voda, Nr 41A, Iasi 700487, Romania.
Carbohydr Polym. 2013 Oct 15;98(1):341-8. doi: 10.1016/j.carbpol.2013.06.009. Epub 2013 Jun 15.
Response surface methodology was used to optimize cellulose recovery from orange peel using sodium hydroxide (NaOH) as isolation reagent, and to minimize its ash content using ethylenediaminetetraacetic acid (EDTA) as chelating agent. The independent variables were NaOH charge, EDTA charge and cooking time. Other two constant parameters were cooking temperature (98 °C) and liquid-to-solid ratio (7.5). The dependent variables were cellulose yield and ash content. A second-order polynomial model was used for plotting response surfaces and for determining optimum cooking conditions. The analysis of coefficient values for independent variables in the regression equation showed that NaOH and EDTA charges were major factors influencing the cellulose yield and ash content, respectively. Optimum conditions were defined by: NaOH charge 38.2%, EDTA charge 9.56%, and cooking time 317 min. The predicted cellulose yield was 24.06% and ash content 0.69%. A good agreement between the experimental values and the predicted was observed.
采用响应面法优化了用氢氧化钠（NaOH）作为分离试剂从橙皮中提取纤维素，并使用乙二胺四乙酸（EDTA）作为螯合剂来最小化其灰分含量。自变量为 NaOH 用量、EDTA 用量和蒸煮时间。其他两个常数参数是蒸煮温度（98°C）和液固比（7.5）。因变量为纤维素得率和灰分含量。采用二次多项式模型绘制响应面并确定最佳蒸煮条件。回归方程中自变量的系数值分析表明，NaOH 和 EDTA 的用量分别是影响纤维素产率和灰分含量的主要因素。最佳条件为：NaOH 用量 38.2%，EDTA 用量 9.56%，蒸煮时间 317 分钟。预测的纤维素得率为 24.06%，灰分含量为 0.69%。实验值与预测值吻合较好。
