Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, Via Tronto 10-A, IT-60126 Ancona, Italy.
Carbohydr Polym. 2013 Oct 15;98(1):665-76. doi: 10.1016/j.carbpol.2013.06.044. Epub 2013 Jun 26.
The idea of using chitosan as a functional delivery aid to support simultaneously PRP, stem cells and growth factors (GF) is associated with the intention to use morphogenic biomaterials to modulate the natural healing sequence in bone and other tissues. For example, chitosan-chondroitin sulfate loaded with platelet lysate was included in a poly(D,L-lactate) foam that was then seeded with human adipose-derived stem cells and cultured in vitro under osteogenic stimulus: the platelet lysate provided to the bone tissue the most suitable assortment of GF which induces the osteogenic differentiation of the mesenchymal stem cells. PDGF, FGF, IGF and TGF-β were protagonists in the repair of callus fractures. The release of GF from the composites of chitosan-PRP and either nano-hydroxyapatite or tricalcium phosphate was highly beneficial for enhancing MSC proliferation and differentiation, thus qualifying chitosan as an excellent vehicle. A number of biochemical characteristics of chitosan exert synergism with stem cells in the regeneration of soft tissues.
将壳聚糖用作功能递送助剂来同时支持 PRP、干细胞和生长因子 (GF) 的想法与使用形态发生生物材料来调节骨和其他组织中的自然愈合过程的意图有关。例如，负载血小板裂解物的壳聚糖-硫酸软骨素被包含在聚（D，L-乳酸）泡沫中，然后用人类脂肪来源的干细胞接种，并在成骨刺激下体外培养：血小板裂解物为骨组织提供了最合适的生长因子组合，诱导间充质干细胞的成骨分化。PDGF、FGF、IGF 和 TGF-β 是修复骨痂骨折的主角。壳聚糖-PRP 与纳米羟基磷灰石或磷酸三钙的复合材料中 GF 的释放非常有利于增强 MSC 的增殖和分化，从而使壳聚糖成为一种极好的载体。壳聚糖的许多生化特性与干细胞协同作用，促进软组织再生。
