Department of Biology, Artvin Coruh University Faculty of Science and Arts , Artvin , Turkey .
Pharm Biol. 2013 Dec;51(12):1531-7. doi: 10.3109/13880209.2013.801994. Epub 2013 Aug 29.
The aqueous extracts of Cetraria islandica (L.) Ach. (Parmeliaceae) is traditionally used in many countries against a number of conditions, including inflammatory conditions.
The present study aimed to assess, for the first time, the effectiveness of C. islandica in cultured primary blood cells of Type 1 diabetes subjects.
Diabetic and control blood samples were treated with or without aqueous lichen extract (5 and 10 μg mL(-1)) for 48 h. The activity of antioxidant enzymes in erythrocytes and also malondialdehyde levels in plasma were determined to evaluate the oxidative status. DNA damages were analyzed by SCE, MN and comet assays in cultured human lymphocytes. Additionally, proliferation index (PI) was evaluated in peripheral blood lymphocytes.
There were significant increases in observed total DNA damage (comet assay) (240.2%) and SCE (168.8%), but not in MN frequencies of cultures with diabetes as compared (p > 0.05) to controls. Whereas, the significant reductions of total DNA damage (69.2 and 65.3%) and SCE frequencies (17.7 and 12.3%) were determined when the 5 and 10 mg mL(-1) lichen extract was added to the cell culture medium, respectively. However, lichen extract did not completely inhibit the induction of SCEs in lymphocytes of patients with diabetes. C. islandica extract was also useful on PI rates.
In conclusion, the antioxidant role of C. islandica in alleviating diabetes-induced genomic instability and for increasing cell viability was firstly indicated in the present study.
欧洲岩高兰（Parmeliaceae）的水提物在许多国家被传统用于治疗多种疾病，包括炎症性疾病。
本研究旨在首次评估欧洲岩高兰在 1 型糖尿病患者原代血培养细胞中的疗效。
将糖尿病和对照血样用或不用水提lichen 提取物（5 和 10μg/mL）处理 48 小时。通过测定红细胞中的抗氧化酶活性和血浆中的丙二醛水平来评估氧化状态。通过 SCE、MN 和彗星试验分析培养的人淋巴细胞中的 DNA 损伤。此外，还评估了外周血淋巴细胞的增殖指数（PI）。
与对照组相比，糖尿病组的总 DNA 损伤（彗星试验）（240.2%）和 SCE（168.8%）明显增加，但 MN 频率无差异（p＞0.05）。然而，当将 5 和 10mg/mL 的lichen 提取物加入细胞培养基中时，总 DNA 损伤（分别为 69.2%和 65.3%）和 SCE 频率（分别为 17.7%和 12.3%）明显降低。然而，lichen 提取物并不能完全抑制糖尿病患者淋巴细胞中 SCE 的诱导。欧洲岩高兰提取物对 PI 率也有作用。
总之，本研究首次表明，欧洲岩高兰具有抗氧化作用，可减轻糖尿病引起的基因组不稳定性，并提高细胞活力。
