Aslan Ali, Agar Guleray, Alpsoy Lokman, Kotan Elif, Ceker Selcuk
Department of Biology Teacher Training, Ataturk University, Erzurum, Turkey.
Toxicol Ind Health. 2012 Jul;28(6):505-12. doi: 10.1177/0748233711416944. Epub 2011 Oct 10.
In this study, the antigenotoxic and antioxidant effects of Umbilicaria vellea (UME) and Xantho somloensis (XME) extracts were determined using sister chromatid exchange (SCE), micronuclei (MN) assays, and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities and malondialdehyde (MDA) levels against the effects of aflatoxin B(1) (AFB(1))-induced oxidative stress and genotoxicity in human lymphocytes in vitro. The results showed that the frequencies of SCE, MN, and MDA level decreased, but the activities of SOD and GPx increased when 5 μg/mL and 10 μg/mL doses of UME and XME were added to AFB(1)-treated cultures. Also the present results indicate that strong antioxidative and the antigenotoxicity mechanisms of UME and XME are associated with its antioxidant nature.
在本研究中，利用姐妹染色单体交换（SCE）、微核（MN）试验以及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）活性及丙二醛（MDA）水平，测定了脐衣（UME）和索姆洛黄衣（XME）提取物对黄曲霉毒素B1（AFB1）诱导的体外人淋巴细胞氧化应激和遗传毒性作用的抗基因毒性和抗氧化作用。结果显示，当向经AFB1处理的培养物中添加5μg/mL和10μg/mL剂量的UME和XME时，SCE、MN的频率以及MDA水平降低，但SOD和GPx的活性增加。此外，目前的结果表明，UME和XME强大的抗氧化和抗基因毒性机制与其抗氧化性质有关。
