Pediatric Department, Gansu Provincial Hospital, Lanzhou City, 730000, China.
Eur J Intern Med. 2013 Dec;24(8):835-8. doi: 10.1016/j.ejim.2013.07.009. Epub 2013 Aug 26.
Hepatitis B virus-associated glomerulonephritis (HBV-GN) is a kind of immune complex-induced glomerulonephritis. The present study was designed to determine whether Hepatitis B virus (HBV) genotype is associated with glomerulonephritis in north-west Chinese children.
A total of 296 HBV-infected patients were enrolled in this study. The serum of patients was subjected to DNA extraction and the HBV genotypes were determined by PCR.
The results showed that genotype C (49%) was predominant within the subjects, compared to HBV/B (38.5%), B/C recombinant (7.4%) and none B/C (5.1%). The serum tests showed that the changes of Complement 3 (C3) and alanine amino transferase (ALT) levels in the genotype C patients were significantly greater than those in the genotype B patients. The frequency of genotype C in HBV-GN patients was higher than that in non HBV-GN patients (χ2 value=30.239, P<0.001). But, it was not associated with renal dysfunction. Furthermore, The genotype C was associated with high HBV-DNA load (82.9% vs 17.1%, P<0.001), which is seen more frequently in the HBV-GN children (86.3% vs 13.3%, P=0.004).
The genotype C may play a role in HBV-GN children, via favoring HBV replication.
乙型肝炎病毒相关性肾小球肾炎（HBV-GN）是一种免疫复合物诱导的肾小球肾炎。本研究旨在确定乙型肝炎病毒（HBV）基因型是否与中国西北儿童的肾小球肾炎有关。
本研究共纳入 296 例 HBV 感染患者。对患者的血清进行 DNA 提取，并通过 PCR 确定 HBV 基因型。
结果显示，在研究对象中，基因型 C（49%）占优势，其次是 HBV/B（38.5%）、B/C 重组（7.4%）和非 B/C（5.1%）。血清检测显示，基因型 C 患者的补体 3（C3）和丙氨酸氨基转移酶（ALT）水平变化明显大于基因型 B 患者。HBV-GN 患者的基因型 C 频率高于非 HBV-GN 患者（χ2 值=30.239，P<0.001）。但与肾功能不全无关。此外，基因型 C 与高 HBV-DNA 载量（82.9%比 17.1%，P<0.001）相关，在 HBV-GN 儿童中更为常见（86.3%比 13.3%，P=0.004）。
基因型 C 可能通过促进 HBV 复制在 HBV-GN 儿童中发挥作用。
