Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan 430070, China; Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan 430070, China.
Gene. 2013 Nov 15;531(1):53-63. doi: 10.1016/j.gene.2013.08.028. Epub 2013 Aug 27.
11 genes involved in lipid metabolism were cloned from liver of yellow catfish Pelteobagrus fulvidraco, including CPT 1A, CPT 1B, PPARα, PPARγ, SREBP-1, G6PD, 6PGD, FAS, acetyl-CoA ACCa, ACCb, and LPL. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of yellow catfish. mRNA of all eleven genes was present in liver, muscle, mesenteric adipose, ovary and heart, but at varying levels. The present study will facilitate further studies on the regulation of lipid metabolism at the molecular level for the fish species.
从黄颡鱼肝脏中克隆了 11 个与脂质代谢相关的基因，包括 CPT1A、CPT1B、PPARα、PPARγ、SREBP-1、G6PD、6PGD、FAS、乙酰辅酶 A 羧化酶（ACC）a、ACCb 和 LPL。系统进化分析进一步确定了这些基因，证实了黄颡鱼的分类和进化地位。这 11 个基因的 mRNA 均存在于肝脏、肌肉、肠系膜脂肪、卵巢和心脏中，但水平不同。本研究将有助于进一步研究鱼类在分子水平上对脂质代谢的调控。
