Unidade Estratégica de Investigação e Serviços de Biotecnologia e Recursos Genéticos, Instituto Nacional de Investigação Agrária e Veterinária, Av. República, Quinta do Marquês, 2784-505 Oeiras, Portugal.
Dept. Ciências Agrárias e Biológicas, Centro Univ. Norte Espírito Santo, Univ. Federal Espírito Santo, Rodovia BR 101 Norte, Km. 60, Bairro Litorâneo, CEP 29932-540, São Mateus, ES, Brazil.
J Plant Physiol. 2014 Feb 15;171(3-4):243-9. doi: 10.1016/j.jplph.2013.07.007. Epub 2013 Aug 26.
Environmental temperature change may induce modifications in membrane lipid properties and composition, which account for different physiological responses among plant species. Coffee plants, as many tropical species, are particularly sensitive to cold, but genotypes can present differences that can be exploited to improve crop management and breeding. This work intended to highlight the changes promoted by low non-freezing temperatures (chilling) in phospholipid (PL) composition of chloroplast membranes of genotypes from two Coffea species, Coffea arabica cv. Catuaí (moderately tolerant) and Coffea canephora cv. Conilon (Clone 153, more susceptible), and relate them with cold sensitivity differences. Such evaluation was performed considering a gradual temperature decrease, chilling (4 °C) exposure and a recovery period under rewarming conditions. Catuaí presented an earlier acclimation response than Clone 153 (CL 153). It displayed a higher metabolic activity during acclimation (total fatty acids and total PL increases) and chilling (phosphatidylglycerol increases), and an overall better recovery. Catuaí also showed the highest phosphatidylglycerol unsaturation (higher double bond index) after chilling, in contrast with CL 153 (gradual unsaturation decrease). Higher unsaturation degree in Catuaí than in CL 153 was also observed for phosphatidylcholine and phosphatidylinositol, resulting, mainly, from raises in unsaturated C18:2 and C18:3. It is suggested that an enhanced PL synthesis and turnover induced by a gradual cold exposure, as well as unsaturation increases in major PL classes, is related to decreased Catuaí susceptibility to low temperatures and strongly contributes to sustain photosynthetic activity in this genotype under chilling conditions, as reported in previous work by this team.
环境温度变化可能会引起膜脂性质和组成的改变，这解释了不同植物物种之间存在不同的生理响应。咖啡树，像许多热带物种一样，对寒冷特别敏感，但不同基因型之间存在差异，可以利用这些差异来改善作物管理和培育。本工作旨在强调低温（冷胁迫）对两个咖啡品种（阿拉比卡种的 Catuaí 和卡图艾种的 Conilon）叶绿体膜磷脂（PL）组成的影响，并将这些影响与冷敏感差异联系起来。这种评估是在逐渐降低温度、冷胁迫（4°C）暴露和在升温条件下恢复期间进行的。Catuaí 比 Clone 153 表现出更早的适应响应。在适应期（总脂肪酸和总 PL 增加）和冷胁迫期（磷脂酰甘油增加），它表现出更高的代谢活性，整体恢复更好。冷胁迫后，Catuaí 的磷脂酰甘油不饱和程度（更高的双键指数）也最高，而 Clone 153 则逐渐不饱和程度降低。与 Clone 153 相比，Catuaí 的磷脂酰胆碱和磷脂酰肌醇的不饱和程度也更高，主要是由于 C18:2 和 C18:3 的不饱和程度增加。研究小组此前的研究报告表明，逐渐冷胁迫诱导的 PL 合成和周转增加，以及主要 PL 类别的不饱和程度增加，与 Catuaí 对低温的敏感性降低有关，并有助于在冷胁迫条件下维持该基因型的光合作用活性。
