Somsak Voravuth, Jaihan Ubonwan, Srichairatanakool Somdet, Uthaipibull Chairat
Department of Clinical Chemistry, Faculty of Medical Technology, Western University, Kanchanaburi 71170, Thailand.
Parasitol Int. 2013 Dec;62(6):548-51. doi: 10.1016/j.parint.2013.08.004. Epub 2013 Aug 27.
Impairment of renal function from oxidative stress during malaria infection is one of the leading causes of death in endemic areas. Since blood urea nitrogen and creatinine levels in plasma can be used as markers for monitoring renal damage, this study investigated the effect of green tea extract on reduction of blood urea nitrogen and creatinine levels during malaria infection using Plasmodium berghei ANKA infected mice as in vivo model. For in vivo testing, ICR mice were infected with 1 × 10(7) parasitized erythrocytes and green tea extract was subsequently administered orally twice a day for 10 consecutive days. Parasitemia was estimated by standard microscopy, and blood urea nitrogen and creatinine levels in plasma were also measured. It was found that parasitemia kept increasing until animal death, and is strongly correlated with high blood urea nitrogen and creatinine. The highest levels of blood urea nitrogen and creatinine in plasma were found on day 10 after infection. However, blood urea nitrogen and creatinine levels in plasma were reduced and decreased significantly (p<0.01) in green tea extract treated mice, compared with untreated group. It can be concluded that green tea extract can protect and maintain renal function during malaria infection, and this extract can be developed for use as a supplement and combination therapy.
疟疾感染期间氧化应激导致的肾功能损害是流行地区主要死因之一。由于血浆中的血尿素氮和肌酐水平可作为监测肾损伤的标志物，本研究以感染伯氏疟原虫ANKA的小鼠为体内模型，研究了绿茶提取物对降低疟疾感染期间血尿素氮和肌酐水平的作用。对于体内试验，将ICR小鼠感染1×10⁷个寄生红细胞，随后连续10天每天口服两次绿茶提取物。通过标准显微镜检查估计疟原虫血症，并测量血浆中的血尿素氮和肌酐水平。结果发现，疟原虫血症一直增加直至动物死亡，且与高血尿素氮和肌酐密切相关。感染后第10天血浆中的血尿素氮和肌酐水平最高。然而，与未处理组相比，绿茶提取物处理的小鼠血浆中的血尿素氮和肌酐水平降低且显著下降（p<0.01）。可以得出结论，绿茶提取物可在疟疾感染期间保护和维持肾功能，且该提取物可开发用作补充剂和联合疗法。
