飞秒数字无透镜全息显微镜用于生物样本成像。

Femtosecond digital lensless holographic microscopy to image biological samples.

机构信息

Institut de Noves Tecnologies de la Imatge (INIT), Universitat Jaume I, 12071 Castelló, Spain.

出版信息

Opt Lett. 2013 Sep 1;38(17):3205-7. doi: 10.1364/OL.38.003205.

DOI:10.1364/OL.38.003205

Abstract

The use of femtosecond laser radiation in digital lensless holographic microscopy (DLHM) to image biological samples is presented. A mode-locked Ti:Sa laser that emits ultrashort pulses of 12 fs intensity FWHM, with 800 nm mean wavelength, at 75 MHz repetition rate is used as a light source. For comparison purposes, the light from a light-emitting diode is also used. A section of the head of a drosophila melanogaster fly is studied with both light sources. The experimental results show very different effects of the pinhole size on the spatial resolution with DLHM. Unaware phenomena on the field of the DLHM are analyzed.

摘要

本文介绍了飞秒激光辐射在数字无透镜全息显微镜（DLHM）中对生物样本成像的应用。使用了一台锁模钛宝石激光器作为光源，该激光器发射 12fs 强度半高全宽、800nm 平均波长、75MHz 重复率的超短脉冲。为了进行比较，还使用了发光二极管的光。使用这两种光源研究了果蝇头部的一个切片。实验结果表明，在 DLHM 中，针孔大小对空间分辨率有非常不同的影响。分析了 DLHM 领域中一些未知的现象。