Department of Bioengineering, University of Washington, Seattle, Washington 98105, USA.
Opt Lett. 2013 Sep 1;38(17):3398-401. doi: 10.1364/OL.38.003398.
We present theoretical and experimental results of axial displacement of objects relative to a fixed condenser focal plane (FP) in optical projection tomographic microscopy (OPTM). OPTM produces three-dimensional, reconstructed images of single cells from two-dimensional projections. The cell rotates in a microcapillary to acquire projections from different perspectives where the objective FP is scanned through the cell while the condenser FP remains fixed at the center of the microcapillary. This work uses a combination of experimental and theoretical methods to improve the OPTM instrument design.
我们展示了光学投影层析显微镜（OPTM）中物体相对于固定聚光器焦平面（FP）的轴向位移的理论和实验结果。OPTM 从二维投影生成单细胞的三维重构图像。细胞在微管中旋转以从不同视角获取投影，其中物镜 FP 在穿过细胞时进行扫描，而聚光器 FP 则固定在微管中心。这项工作结合了实验和理论方法来改进 OPTM 仪器设计。
