逆转录病毒介导的编码整合膜蛋白的腺病毒基因转移足以下调表皮生长因子受体。
Retrovirus-mediated transfer of an adenovirus gene encoding an integral membrane protein is sufficient to down regulate the receptor for epidermal growth factor.
作者信息
Hoffman B L, Ullrich A, Wold W S, Carlin C R
机构信息
Institute for Molecular Virology, St. Louis University Medical Center, Missouri 63110.
出版信息
Mol Cell Biol. 1990 Oct;10(10):5521-4. doi: 10.1128/mcb.10.10.5521-5524.1990.
Abstract
We have used retrovirus-mediated gene transfer to introduce sequences encoding a 10,400-molecular-weight (10.4K) adenovirus protein previously shown to down regulate the receptor for epidermal growth factor (EGF) into two murine cell lines that possess human EGF receptors (EGF-Rs). Assays for receptor expression showed that acute infection resulted in rapid, constitutive down regulation of the EGF-R via a pathway that appears to be endosome mediated. This represents the first demonstration that 10.4K expression in the absence of other virus-encoded proteins is sufficient to elicit this response. The usefulness of this approach for the study of 10.4K-mediated signal transduction in cells with a nontransformed phenotype is discussed.
摘要
我们利用逆转录病毒介导的基因转移，将编码一种分子量为10400（10.4K）的腺病毒蛋白的序列导入两种具有人表皮生长因子受体（EGF-Rs）的小鼠细胞系中，该蛋白先前已被证明可下调表皮生长因子（EGF）受体。受体表达分析表明，急性感染通过一种似乎由内体介导的途径导致EGF-R的快速、组成性下调。这首次证明，在没有其他病毒编码蛋白的情况下，10.4K蛋白的表达足以引发这种反应。本文讨论了这种方法在研究具有非转化表型的细胞中10.4K介导的信号转导方面的实用性。
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1
Retrovirus-mediated transfer of an adenovirus gene encoding an integral membrane protein is sufficient to down regulate the receptor for epidermal growth factor.逆转录病毒介导的编码整合膜蛋白的腺病毒基因转移足以下调表皮生长因子受体。
Mol Cell Biol. 1990 Oct;10(10):5521-4. doi: 10.1128/mcb.10.10.5521-5524.1990.
2
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