Zhu Fenxia, Chen Jiaquan, Wang Jingjing, Yin Rong, Li Xiufeng, Jia Xiaobin
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, PR China
School of Sciences, China Pharmaceutical University, Nanjing 210009, PR China.
J Chromatogr Sci. 2014 Sep;52(8):862-71. doi: 10.1093/chromsci/bmt129. Epub 2013 Aug 28.
An ultra performance liquid chromatography coupled with photodiode array detector and time-of-flight tandem mass spectrometry (UPLC-PDA-TOF-MS) method was developed for the quality assessment of Danmu preparations, a commonly used traditional Chinese medicine. Thirty-three compounds from Danmu preparations were simultaneously detected; among them, 14 compounds were unequivocally identified based on their retention behaviors, UV spectrum, MS and MS(n) data by comparing with reference substances, and the others were tentatively characterized by literatures. Twelve of 33 compounds were simultaneously determined by UPLC-PDA, and the validation of the quantitative method, including recoveries, linearity, sensitivity, precision and repeatability was carried out and the results demonstrated to be satisfied the requirements of quantitative analysis. The results suggested that the established method would be a powerful and reliable analytical tool for quality control of Danmu preparations and the characterization of multi-constituent in complex chemical system.
建立了一种超高效液相色谱-光电二极管阵列检测器-飞行时间串联质谱联用（UPLC-PDA-TOF-MS）方法用于常用中药丹木制剂的质量评价。从丹木制剂中同时检测出33种化合物；其中，通过与对照品比较保留行为、紫外光谱、质谱和质谱（n）数据，明确鉴定了14种化合物，其余化合物通过文献初步表征。采用UPLC-PDA同时测定了33种化合物中的12种，并对定量方法进行了回收率、线性、灵敏度、精密度和重复性等验证，结果表明该方法满足定量分析要求。结果表明，所建立的方法将是丹木制剂质量控制和复杂化学体系中多成分表征的有力可靠分析工具。
