豌豆rbcS-3A上游区域内一个光敏色素反应元件的定位
Localization of a phytochrome-responsive element within the upstream region of pea rbcS-3A.
作者信息
Gilmartin P M, Chua N H
机构信息
Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10021-6399.
出版信息
Mol Cell Biol. 1990 Oct;10(10):5565-8. doi: 10.1128/mcb.10.10.5565-5568.1990.
Abstract
The pea rbcS-3A promoter with a 5' deletion to -166 (-166 rbcS-3A) contains two GT-1-binding sites. Mutational analyses demonstrated that a decrease in affinity for GT-1 correlates with reduced promoter activity. Transcription of -166 rbcS-3A in transgenic etiolated seedlings is induced by red light and suppressed by far-red light, indicating that it contains a phytochrome-responsive element.
摘要
具有 5' 端缺失至 -166 的豌豆 rbcS-3A 启动子（-166 rbcS-3A）包含两个 GT-1 结合位点。突变分析表明，对 GT-1 的亲和力降低与启动子活性降低相关。-166 rbcS-3A 在转基因黄化幼苗中的转录受红光诱导并被远红光抑制，表明它含有一个光敏色素反应元件。
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