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丁型肝炎病毒RNA自我切割的RNA构象要求

RNA conformational requirements of self-cleavage of hepatitis delta virus RNA.

作者信息

Wu H N, Lai M M

机构信息

Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Republic of China.

出版信息

Mol Cell Biol. 1990 Oct;10(10):5575-9. doi: 10.1128/mcb.10.10.5575-5579.1990.

DOI:10.1128/mcb.10.10.5575-5579.1990
PMID:2398903
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC361278/
Abstract

Hepatitis delta virus (HDV) RNA subfragments undergo self-cleavage at varying efficiencies. We have developed a procedure of using repeated cycles of heat denaturation and renaturation of RNA to achieve a high efficiency of cleavage. This effect can also be achieved by gradual denaturation of RNA with heat or formamide. These results suggest that only a subpopulation of the catalytic RNA molecules assumes the active conformation required for self-cleavage. This procedure could be of general use for detecting catalytic RNA activities.

摘要

丁型肝炎病毒（HDV）RNA亚片段以不同效率进行自我切割。我们开发了一种利用RNA反复热变性和复性循环的程序来实现高效切割。通过用热或甲酰胺使RNA逐渐变性也可达到这种效果。这些结果表明，只有一部分催化RNA分子呈现自我切割所需的活性构象。该程序可普遍用于检测催化RNA活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6003/361278/faee57e6b586/molcellb00046-0560-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6003/361278/faee57e6b586/molcellb00046-0560-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6003/361278/faee57e6b586/molcellb00046-0560-a.jpg

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RNA conformational requirements of self-cleavage of hepatitis delta virus RNA.丁型肝炎病毒RNA自我切割的RNA构象要求
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本文引用的文献

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Autolytic processing of dimeric plant virus satellite RNA.二聚体植物病毒卫星 RNA 的自溶加工。
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Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.利用热力学和辅助信息对大型RNA序列进行最优计算机折叠
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Controlling mammalian gene expression by allosteric hepatitis delta virus ribozymes.通过变构性丁型肝炎病毒核酶控制哺乳动物基因表达。
ACS Synth Biol. 2013 Dec 20;2(12):684-9. doi: 10.1021/sb400037a. Epub 2013 May 22.
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Nucleic acid binding properties of the nucleic acid chaperone domain of hepatitis delta antigen.丁型肝炎抗原核酸伴侣结构域的核酸结合特性
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Nucleic Acids Res. 1981 Jan 10;9(1):133-48. doi: 10.1093/nar/9.1.133.
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