Zhang Zhenzhen, Wang Yicui, Shi Zhubing, Zhang Min
School of Life Sciences, Anhui University, Hefei, Anhui 230039, People's Republic of China.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep;69(Pt 9):989-93. doi: 10.1107/S1744309113021520. Epub 2013 Aug 23.
MO25, a conserved scaffold protein, activates the tumour suppressor LKB1 with the pseudokinase STRAD. MO25 also promotes the activities of the STE20-family kinases MST3, MST4, STK25, SPAK and OSR1. Zebrafish MO25 was purified and crystallized, and a crystal of zebrafish MO25 diffracted to 2.9 Å resolution and belonged to space group P3221, with unit-cell parameters a = b = 156.665, c = 221.251 Å. The structure of zebrafish MO25 was determined by molecular replacement. It is constituted of seven helical repeats. Structural comparison indicates that the overall structures of zebrafish and human MO25 are very similar, suggesting that MO25 has conserved functions in zebrafish. This work provides a structural basis for further functional and evolutionary studies of MO25.
MO25是一种保守的支架蛋白，它与假激酶STRAD一起激活肿瘤抑制因子LKB1。MO25还能促进STE20家族激酶MST3、MST4、STK25、SPAK和OSR1的活性。斑马鱼MO25被纯化并结晶，斑马鱼MO25的晶体衍射分辨率达到2.9 Å，属于P3221空间群，晶胞参数a = b = 156.665，c = 221.251 Å。斑马鱼MO25的结构通过分子置换法确定。它由七个螺旋重复序列组成。结构比较表明，斑马鱼和人类MO25的整体结构非常相似，这表明MO25在斑马鱼中具有保守功能。这项工作为MO25进一步的功能和进化研究提供了结构基础。
