Choudhury Rimjhim Roy, Basak Supriyo, Ramesh Aadi Moolam, Rangan Latha
Department of Biotechnology, Indian Institute of Technology Guwahati, Assam, 781 039, India.
Protoplasma. 2014 May;251(3):703-9. doi: 10.1007/s00709-013-0545-4. Epub 2013 Aug 29.
Pongamia pinnata L. is a multipurpose versatile legume that is well known as a prospective feedstock biodiesel species. However, to date, there has been little genomic research aimed at the exploitation of the biotechnological potential of this species. Genetic characterization of any plant is a challenging task when there is no information about the genome size and organization of the species. Therefore, the genome size of P. pinnata was estimated by flow cytometry with respect to two standards (Zea mays and Pisum sativum), and compared with that of in vitro-raised plants (nodal segment, in vitro-rooted plantlets and acclimatized in vitro plants) to study the potential effect of somaclonal variation on genome size. This method can be used to support the establishment of true-to-type plants to encourage afforestation programs. Modified propidium iodide/hypotonic citrate buffer was used for isolation of the intact nuclei. The 2C DNA value of this species was estimated to be 2.51 ± 0.01 pg. Statistically, there was no significant difference in the DNA content of the in vitro-grown plants and mother plant at α = 0.05. As a result of the low genome size of P. pinnata, a species that has adapted itself to a wide range of edaphic and ecological condition, we can now proceed for its next generation sequencing and genomic diversity studies.
水黄皮是一种多用途的豆科植物，作为一种潜在的生物柴油原料物种而广为人知。然而，迄今为止，针对该物种生物技术潜力开发的基因组研究很少。当没有关于某个物种基因组大小和组织的信息时，对任何植物进行遗传特征分析都是一项具有挑战性的任务。因此，采用流式细胞术以两种标准（玉米和豌豆）估计了水黄皮的基因组大小，并与离体培养植物（茎段、离体生根苗和驯化后的离体植物）进行比较，以研究体细胞克隆变异对基因组大小的潜在影响。该方法可用于支持建立与原种一致的植物，以推动造林计划。使用改良的碘化丙啶/低渗柠檬酸盐缓冲液分离完整的细胞核。该物种的2C DNA值估计为2.51±0.01 pg。在α=0.05时，离体培养植物和母本植物的DNA含量在统计学上没有显著差异。由于水黄皮基因组大小较低，且该物种已适应广泛的土壤和生态条件，我们现在可以进行其下一代测序和基因组多样性研究。
